Following fixation in 4% PFA, the cells were assessed for Sall4 expression using 1:100 dilution of the primary antibody (ab29112) in 1% serum, 0.1% triton, 0.1% BSA in PBS, followed by detection using goat polyclonal rabbit IgG Alexa 488 (ab150077) at 1:200. The results show that nuclear Sall4 (green) was clearly observed. An isotype control IgG was run in parallel and showed no positive staining (not shown here).
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Submitted Aug 21 2013
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