Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) (ab150079)
- Datasheet
- Specific References (30)
- Protocols
Overview
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Product nameGoat Anti-Rabbit IgG H&L (Alexa Fluor® 647)
See all IgG secondary antibodies -
DescriptionGoat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 647)
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Host speciesGoat
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Target speciesRabbit
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SpecificityThis antibody is specific to Rabbit IgG
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Tested applicationsSuitable for: IHC-Fr, ICC/IF, ELISA, IHC-P, Flow Cytmore details
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Immunogen
The details of the immunogen for this antibody are not available.
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ConjugationAlexa Fluor® 647. Ex: 652nm, Em: 668nm
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.02% Sodium azide
Constituents: PBS, 30% Glycerol, 1% BSA -
Concentration information loading... -
PurityImmunogen affinity purified
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Purification notesThis antibody was isolated by affinity chromatography using antigen coupled to agarose beads.
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ClonalityPolyclonal
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IsotypeIgG
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General notes
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to use products containing Alexa Fluor® dyes for purposes other than research, contact Life Technologies Corporation, 5791 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@lifetech.com.
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Research areas
Associated products
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Related Buffer
Applications
Our Abpromise guarantee covers the use of ab150079 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| IHC-Fr | Use at an assay dependent concentration. | |
| ICC/IF | 1/200 - 1/1000. | |
| ELISA | Use at an assay dependent concentration. | |
| IHC-P | Use at an assay dependent concentration. | |
| Flow Cyt | 1/2000. |
Images
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Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) (ab150079)ICC/IF image of ab6046 in HeLa cells. The cells were 100% methanol fixed (5 min), permeabilized with 0.1% Triton X-100 for 5 minutes and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab6046, 2µg/ml) overnight at +4°C. The secondary antibody ab150079 (shown in red) was used at 1µg/ml for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
The negative control (inset) is a secondary-only assay to demonstrate low non-specific binding of the secondary antibody.
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Alexa Fluor® - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 647) (ab150079)
References
This product has been referenced in:
- Zhang Y et al. Clostridium difficile toxin B recombinant protein inhibits tumor growth and induces apoptosis through inhibiting Bcl-2 expression, triggering inflammatory responses and activating C-erbB-2 and Cox-2 expression in breast cancer mouse model. Biomed Pharmacother 101:391-398 (2018). Read more (PubMed: 29501042) »
- Zeng J et al. Tumor-associated macrophages recruited by periostin in intrahepatic cholangiocarcinoma stem cells. Oncol Lett 15:8681-8686 (2018). Read more (PubMed: 29805605) »
Customer reviews and Q&As
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"