• Product name
    Goat Anti-Rabbit IgG H&L (HRP)
    See all IgG secondary antibodies
  • Host species
  • Target species
  • Specificity
    By immunoelectrophoresis and ELISA this antibody reacts specifically with Rabbit IgG and with light chains common to other Rabbit immunoglobulins. No antibody was detected against non-immunoglobulin serum proteins.
  • Tested applications
    Suitable for: ICC, IHC-P, ELISA, WBmore details
  • Conjugation


  • Form
  • Storage instructions
    Shipped at 4°C. Store at +4°C.
  • Storage buffer
    Preservative: 0.1% Proclin
    Constituents: 0.2% BSA, PBS
  • Concentration information loading...
  • Purity
    Immunogen affinity purified
  • Purification notes
    This antibody was isolated by affinity chromatography using antigen coupled to agarose beads and conjugated to Horse Radish Peroxidase (HRP).
  • Conjugation notes
    Molar enzyme/ antibody protein ratio is 4:1
  • Clonality
  • Isotype
  • General notes
    Part of the AbExcel range.
  • Research areas


Our Abpromise guarantee covers the use of ab97051 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC Use at an assay dependent dilution.
IHC-P 1/200 - 1/5000.
ELISA 1/10000 - 1/100000. (Primary).
WB 1/2000 - 1/20000. Colorimetric: 1/5000 - 1/30000; Chemiluminescent: 1/10000 - 1/50000


  • IHC image of beta Actin staining in normal human colon, formalin-fixed and paraffin-embedded tissue*. The section was pre-treated using pressure cooker heat mediated antigen retrieval with sodium citrate buffer (pH6) for 30mins. The section was incubated with ab8227, 3µg/ml overnight at +4°C. An HRP-conjugated secondary (Ab97051, 1/2000 dilution) was used for 1hr at room temperature. The section was counterstained with haematoxylin and mounted with DPX.

    The inset negative control image is secondary-only at 1/500 dilution.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • All lanes : Anti-beta Actin antibody (ab8227) at 1 µg/ml

    All lanes : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 10 µg per lane.

    Lanes 1-2 : Rabbit polyclonal to GNAT2 (ab97501) at 1/2000 dilution
    Lanes 3-4 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
    Lanes 5-6 : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Exposure time: 10 seconds



This product has been referenced in:
  • Singh G  et al. Genome-wide profiling of the PIWI-interacting RNA-mRNA regulatory networks in epithelial ovarian cancers. PLoS One 13:e0190485 (2018). Read more (PubMed: 29320577) »
  • Liang Z & Ren C Emodin attenuates apoptosis and inflammation induced by LPS through up-regulating lncRNA TUG1 in murine chondrogenic ATDC5 cells. Biomed Pharmacother 103:897-902 (2018). WB . Read more (PubMed: 29710506) »
See all 157 Publications for this product

Customer reviews and Q&As

1-10 of 36 Abreviews or Q&A


Gracias por tu respuesta. Me alegra oír que este secundario os ha dado buenos resultados.

Ya he realizado por tanto el nuevo pedido completamente gratuito del mismo anticuerpo: ab97051.

Recibiréis una confirmación automática del mismo con más detalles.

Si necesitáis cualquier información referente al pedido, no dudéis en contactarnos, referenciando el número de éste: XXXXX.

Si tuvierais cualquier otro problema, o requirierais mas información / asistencia, por favor, no dudéis en volvernos a contactar.

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Thank you for passing some information to me.
I understand that ab3583 has been used previously at 1:4000.
Have you had a chance to test this antibody at dilutions of 1/500 or 1/1000 (for 1 hr at room temperature or overnight at 4oC?
Would you prefer to get a new vial of the primary antibody? Please do let me know how you wish to proceed.
I look forward to hearing from you soon.

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Western blot
This antibody works well for western blotting assay. The antigen I detected in the attached blot is 42 kD, the result looks great with this antibody (1:5,000 dilution).

Abcam user community

Verified customer

Submitted Sep 27 2018

Western blot
this antibody works stably at a dilution of 1:4,000 for western blot assay.

Abcam user community

Verified customer

Submitted Feb 08 2016

Western blot
always works for WB at a dilution of 1:5,000.

Abcam user community

Verified customer

Submitted Nov 02 2015

Western blot
We stock and use this for many of our westerns, gets the job done!
("B" and "G" are brain and gonad samples.)

Sebastian Alvarado

Verified customer

Submitted Aug 21 2015

Western blot
1. The concentration of Goat Anti-Rabbit IgG H&L (HRP) used for this experiment is 1:5000 and incubated for 2 hours.
2. Proteins were blocked with 5% BSA in TBS with 0.1% TritonX-100.

Mr. Lenin Veeraval

Verified customer

Submitted Jun 18 2015

Western blot
Abcam's anti-rabbit HRP is very reliable and economical to use. We ended up using 10x less than our old antibody from a different company. Since it is so robust, we started calling it the "epic rabbit antibody" to distinguish it from the old one we have in the lab. The lower band in the gel corresponds to beta-tubulin (ab6046) followed by anti-rabbit HRP (ab97051) while the upper band is a different 70kDa protein followed by anti-mouse HRP (ab97023).

Abcam user community

Verified customer

Submitted Aug 21 2014

Western blot
Secondary worked very well. Used at 1:5000.

Abcam user community

Verified customer

Submitted Aug 15 2014


This product has been tested using ECL and will perform very well using this detection system.

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1-10 of 36 Abreviews or Q&A


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