Recombinant Anti-Granzyme B antibody [EPR8260] (ab134933)
![Flow Cytometry - Anti-Granzyme B antibody [EPR8260] (ab134933)](https://www.abcam.com/ps/products/134/ab134933/Images/ab134933-482698-AntiGranzyme-B-antibody-EPR8260-flow-cyomtery-nogfpcd16nk92.jpg "Flow Cytometry - Anti-Granzyme B antibody [EPR8260] (ab134933)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR8260] to Granzyme B
- Suitable for: WB, IHC-P, Flow Cyt
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-Granzyme B antibody [EPR8260]
See all Granzyme B primary antibodies -
Description
Rabbit monoclonal [EPR8260] to Granzyme B -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, Flow Cytmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- IHC-P: Human Hodgkins lymphoma and Human tonsil tissues WB: KARPAS-299, SR whole cell lysates; Flow cyt: No-GFP-CD16.NK-92 cells
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
Dissociation constant (KD)
KD = 7.80 x 10 -11 M Learn more about KD -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR8260 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
- Anti-Granzyme B antibody [EPR8260] - Low endotoxin, Azide free (ab214443)
- Alexa Fluor® 647 Anti-Granzyme B antibody [EPR8260] (ab225185)
- Anti-Granzyme B antibody [EPR8260] - BSA and Azide free (ab226162)
- Alexa Fluor® 555 Anti-Granzyme B antibody [EPR8260] (ab280851)
- Anti-Granzyme B antibody [EPR8260] - Rat IgG2a (ab289888)
- Anti-Granzyme B antibody [EPR8260] - Chimeric – BSA and Azide free (ab289895)
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Assay kits
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab134933 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/1000. Predicted molecular weight: 28 kDa.
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| IHC-P | (2) |
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
For antigen retrieval: heat up to 98 degrees C, below boiling, and then let cool for 10-20 min. Use of an HRP/AP polymerized secondary antibody is recommended. |
| Flow Cyt |
1/100.
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| Notes |
|---|
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WB
1/1000. Predicted molecular weight: 28 kDa. |
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IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. For antigen retrieval: heat up to 98 degrees C, below boiling, and then let cool for 10-20 min. Use of an HRP/AP polymerized secondary antibody is recommended. |
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Flow Cyt
1/100. |
Target
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Function
This enzyme is necessary for target cell lysis in cell-mediated immune responses. It cleaves after Asp. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis. -
Sequence similarities
Belongs to the peptidase S1 family. Granzyme subfamily.
Contains 1 peptidase S1 domain. -
Cellular localization
Cytoplasmic granule. Cytoplasmic granules of cytolytic T-lymphocytes and natural killer cells. - Information by UniProt
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Database links
- Entrez Gene: 3002 Human
- Omim: 123910 Human
- SwissProt: P10144 Human
- Unigene: 1051 Human
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Alternative names
- C11 antibody
- Cathepsin G like 1 antibody
- Cathepsin G-like 1 antibody
see all
Images
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Flow Cytometry - Anti-Granzyme B antibody [EPR8260] (ab134933)
Flow cytometric analysis of 4% paraformaldehyde fixed No-GFP-CD16.NK-92 Human Natural Killer cells labeling Granzyme B with ab134933 at 1/100 dilution (Red) compared with an Isotype control Rabbit monoclonal IgG (ab172730) (Black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). Goat anti rabbit IgG Alexa Fluor® 488 (ab150081), at 1/5000 dilution was used as the secondary antibody.
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![Western blot - Anti-Granzyme B antibody [EPR8260] (ab134933)](https://www.abcam.com/ps/products/134/ab134933/Images/ab134933-469826-anti-granzyme-b-antibody-karpas-299-jurkat-sr-human.jpg)
Western blot - Anti-Granzyme B antibody [EPR8260] (ab134933)All lanes : Anti-Granzyme B antibody [EPR8260] (ab134933) at 1/1000 dilution
Lane 1 : KARPAS-299 (human anaplastic large cell lymphoma), whole cell lysate
Lane 2 : Jurkat (human T cell leukemia T lymphocyte), whole cell lysate
Lane 3 : SR (human pleural effusion lymphoblast, whole cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Predicted band size: 28 kDa
Observed band size: 28 kDa
Exposure time: 48 secondsNegative control: Jurkat (PMID:18437383, 25168906).
Blocking and diluting buffer: 5% NFDM/TBST
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme B antibody [EPR8260] (ab134933)](https://www.abcam.com/ps/products/134/ab134933/Images/ab134933-330505-anti-granzyme-b-antibody-epr8260-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme B antibody [EPR8260] (ab134933)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human tonsil tissue sections labeling Granzyme B with Purified ab134933 at 1:250 dilution (2.96 µg/ml). Heat mediated antigen retrieval was performed using ab93684 (Tris/EDTA buffer, pH 9.0). ImmunoHistoProbe one step HRP Polymer (ready to use)was used as the secondary antibody. Negative control:PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme B antibody [EPR8260] (ab134933)](https://www.abcam.com/ps/products/134/ab134933/Images/ab134933-149118-anti-granzyme-b-antibody-epr8260-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme B antibody [EPR8260] (ab134933)Immunohistochemical analysis of paraffin-embedded, formalin-fixed Human Hodgkin's lymphoma tissue, labelling Granzyme B using unpurified ab134933 at a 1/100 dilution. Heat mediated antigen retrieval was performed with citrate buffer pH 6 before commencing with IHC staining protocol.
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![OI-RD Scanning - Anti-Granzyme B antibody [EPR8260] (ab134933)](https://www.abcam.com/ps/products/134/ab134933/Images/ab134933-149117-anti-granzyme-b-antibody-epr8260.jpg)
OI-RD Scanning - Anti-Granzyme B antibody [EPR8260] (ab134933) -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme B antibody [EPR8260] (ab134933)](https://www.abcam.com/ps/products/134/ab134933/Images/ab134933-10-anti-granzyme-b-antibody-epr8260-tissue-microarray-human-ab134933.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Granzyme B antibody [EPR8260] (ab134933)Tissue Microarrays stained for "Anti-Granzyme B antibody [EPR8260]” using "ab134933" in immunohistochemical analysis. This table provides a detailed overview of positive (tick mark) and negative (cross mark) staining per sample type tested. The sections were pre-treated using Heat mediated antigen retrieval using Bond™ Epitope Retrieval Solution 2 (pH 9.0) for 20 minutes. The sections were incubated with ab134933 for 30 mins at room temperature followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). The immunostaining was performed on a Leica Biosystems BOND® RX instrument.
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![Anti-Granzyme B antibody [EPR8260] (ab134933)](https://www.abcam.com/ps/products/134/ab134933/Images/ab134933-6-benefits-of-recombinant-antibodies.png)
Anti-Granzyme B antibody [EPR8260] (ab134933)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (8)
ab134933 has been referenced in 8 publications.
- Jiang Y et al. Hypoxia enhances the production and antitumor effect of exosomes derived from natural killer cells. Ann Transl Med 9:473 (2021). PubMed: 33850870
- Yu H et al. Acute lymphoblastic leukemia-derived exosome inhibits cytotoxicity of natural killer cells by TGF-ß signaling pathway. 3 Biotech 11:313 (2021). PubMed: 34109098
- Qiu M et al. Modulation of intestinal microbiota by glycyrrhizic acid prevents high-fat diet-enhanced pre-metastatic niche formation and metastasis. Mucosal Immunol 12:945-957 (2019). PubMed: 30755716
- Ottaiano A et al. Cetuximab, irinotecan and fluorouracile in fiRst-line treatment of immunologically-selected advanced colorectal cancer patients: the CIFRA study protocol. BMC Cancer 19:899 (2019). PubMed: 31500586
- Shibutani M et al. A comparison of the local immune status between the primary and metastatic tumor in colorectal cancer: a retrospective study. BMC Cancer 18:371 (2018). IHC-P ; Human . PubMed: 29614981
- Overgaard NH et al. Genetically Induced Tumors in the Oncopig Model Invoke an Antitumor Immune Response Dominated by Cytotoxic CD8ß+ T Cells and Differentiated ?d T Cells Alongside a Regulatory Response Mediated by FOXP3+ T Cells and Immunoregulatory Molecules. Front Immunol 9:1301 (2018). PubMed: 29930558
- García-Hernández ML et al. A Unique Cellular and Molecular Microenvironment Is Present in Tertiary Lymphoid Organs of Patients with Spontaneous Prostate Cancer Regression. Front Immunol 8:563 (2017). IF ; Human . PubMed: 28567040
- da Silva Santos C et al. Proteome profiling of human cutaneous leishmaniasis lesion. J Invest Dermatol 135:400-10 (2015). IHC ; Human . PubMed: 25207817