Western blot abreview for Anti-HA tag antibody - ChIP Grade

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Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (Embryonic kidney fibroblast cells)
Gel Running Conditions
Reduced Denaturing (10% SDS-PAGE)
Loading amount
1e+006 cells
Specification
Embryonic kidney fibroblast cells
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 23°C

Other product details

Incubation time
1 hour(s) and 30 minute(s) · Temperature: 23°C · Diluent: TBST
Dilution
1/1000

Secondary antibody

Dilution
1/5000
Name
Non-Abcam antibody was used: anti-rabbit LICOR
Host species: Goat
Clonality: Monoclonal
Conjugation: IRDye® 680

Detection

Detection method
Odyssey scanner
Negative control
293T cells transfected with HA-vector plasmid
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 73 kDa
Positive control
293T cells transfected with plasmid expressing HA-tagged protein of interest

Additional data

Additional Notes
Mouse anti-HA (H9658) from Sigma and Dynabeads protein-G from Life Technologies were used for the immunoprecipitation experiment. The notations I/P and P/D denote input and pull-down respectively in the figure.

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Submitted Apr 01 2016

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