Western blot abreview for Anti-HA tag antibody - ChIP Grade

Excellent
Abreviews
Application
Western blot
Sample
Human Cell lysate - whole cell (Whole cell lysate resolved on a 12% SDS PAGE gel)
Gel Running Conditions
Reduced Denaturing (12% SDS gel)
Loading amount
25 µg
Treatment
HEK293T cells transfected with HA-Rad21 plasmid for overexpression
Specification
Whole cell lysate resolved on a 12% SDS PAGE gel
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Other product details

Incubation time
3 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: 1X PBS (0.1% Triton X-100) buffer
Dilution
1/2000

Secondary antibody

Dilution
1/10000
Name
Non-Abcam antibody was used: Immun-Star Goat Anti-Rabbit (GAR)-HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL reagent was used
Exposure
5 second(s)
Bands
Specific: 78 kDa

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Submitted Jan 19 2017

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