ChIP abreview for Anti-HA tag antibody - ChIP Grade

Good
Abreviews
Application
ChIP
Sample
Mouse Cell lysate - nuclear (mouse embrynic stem cells)
Negative control
Promoters Sdha and b-Act
Specification
mouse embrynic stem cells
Detection step
Real-time PCR
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 15 minute(s) and 0 second(s)
Specification of the cross-linking agent: Formaldhyde 0.75%
Positive control
Promoters of Cbx8 and Hmox1

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/300

Additional data

Additional Notes
The HA antibody is used against transiently expressed HA-tagged Tif1a protein. The antibody is used at 1.5 ug per 15 millions cells Seems also that this antibody has some background in ChiP.

Abcam user community

Verified customer

Submitted Apr 21 2017

Sign up