Immunocytochemistry/ Immunofluorescence abreview for Anti-HA tag antibody - ChIP Grade

Excellent
Abreviews
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (alveolar type II cell transfected with a HA-Tagged)
Permeabilization
Yes - Saponin
Specification
alveolar type II cell transfected with a HA-Tagged
Blocking step
Serum as blocking agent for 5 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Paraformaldehyde

Other product details

Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: Blocking Buffer
Dilution
1/250

Secondary antibody

Dilution
1/400
Name
Non-Abcam antibody was used: Donkey anti Rabbit 568
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor­ 568

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Submitted Aug 18 2017

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