Overview

  • Product name
    HA tag peptide

Description

  • Nature
    Synthetic
  • Amino Acid Sequence

    Associated products

    Specifications

    Our Abpromise guarantee covers the use of ab13835 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    • Applications

      Blocking - Blocking peptide for Anti-HA tag antibody (ab13834)

    • Form
      Liquid
    • Additional notes

      - First try to dissolve a small amount of peptide in either water or buffer. The more charged residues on a peptide, the more soluble it is in aqueous solutions.
      - If the peptide doesn’t dissolve try an organic solvent e.g. DMSO, then dilute using water or buffer.
      - Consider that any solvent used must be compatible with your assay. If a peptide does not dissolve and you need to recover it, lyophilise to remove the solvent.
      - Gentle warming and sonication can effectively aid peptide solubilisation. If the solution is cloudy or has gelled the peptide may be in suspension rather than solubilised.
      - Peptides containing cysteine are easily oxidised, so should be prepared in solution just prior to use.

    • Concentration information loading...

    Preparation and Storage

    • Stability and Storage

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

      Information available upon request.

    General Info

    • Alternative names
      • HA epitope tag
      • HA1
      • HA2
      • hemagglutinin
      • Hemagglutinin HA1 chain
      • Hemagglutinin HA2 chain
      see all
    • Relevance
      Human influenza hemagglutinin (HA) is a surface glycoprotein required for the infectivity of the human virus. The HA tag is derived from the HA molecule corresponding to amino acids 98-106 has been extensively used as a general epitope tag in expression vectors. Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins.

    Images

    • Western blot using ab13834 on 293 cells transfected with a 55 kDa HA-tagged minigene (ab20896). Lane 1: ab13834 Lane 2: ab13834 with blocking peptide ab13835. Primary: Rabbit polyclonal to HA tag (1/500) Secondary: Alexa Fluor 680 Goat anti Rabbit IgG (1/5000). Lysates at 10µg/lane.

    References

    This product has been referenced in:
    See 1 Publication for this product

    Customer reviews and Q&As

    1-3 of 3 Abreviews or Q&A

    Answer

    Thank you for contacting Abcam regarding ab13835.


    This product is the HA peptide used as the immunogen to generate the antibody ab13834. Since it is only 9aa, it cannot be run on a gel to use as a positive control or standard. Instead, I would recommend a purified HA-tagged protein or a cell lysate containing an HA tagged protein, such as ab20896.


    I hope this information is helpful. Please do not hesitate to contact me if you have any additional questions.

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    Answer

    Thank you for contacting us.
    Yes, we expect that antibody to bind this peptide. The peptide ab13835 does contain the classical HA tag (amino acid 98 to 106 of the surface hemagglutinin glycoprotein of Human influenza virus). The cited antibody is directed against the HA tag and should therefore be able to recongize this peptide ab13835.
    I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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    Answer

    Here is a general protocol for eluting your HA-tagged protein with ab13835, our HA tag peptide: The peptide arrives at a 1mg/ml concentration. After the last wash, remove all of the wash buffer and add a sufficient volume of the peptide for your downstream use. For example, 30-50ul of the peptide might be a useful volume. Elute for at least 1 hour at 4 degrees C with gentle agitation. Pellet the beads and remove the supernatant. Confirm elution of target protein via coomassie staining and/or western blotting.

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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