Overview

  • Product name
    Anti-HADHSC antibody [1A12BC8]
    See all HADHSC primary antibodies
  • Description
    Mouse monoclonal [1A12BC8] to HADHSC
  • Host species
    Mouse
  • Tested applications
    Suitable for: IHC-P, ICC/IF, IP, Flow Cyt, In-Cell ELISAmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Human liver mitochondria

  • Positive control
    • MRC5 fibroblasts; Human cerebellum tissue; Human liver mitochondria; HL-60 cells.
  • General notes

    This antibody clone is manufactured by Abcam.

    Product was previously marketed under the MitoSciences sub-brand.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

Properties

Applications

Our Abpromise guarantee covers the use of ab110284 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/250. Perform heat mediated antigen retrieval - 1 min pressure cook in 1mmol EDTA pH8.
ICC/IF Use a concentration of 5 µg/ml.
IP Use at an assay dependent concentration.
Flow Cyt Use a concentration of 1 µg/ml.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

In-Cell ELISA Use a concentration of 2 µg/ml. (0.2 µg/well).

Target

  • Function
    Plays an essential role in the mitochondrial beta-oxidation of short chain fatty acids. Exerts it highest activity toward 3-hydroxybutyryl-CoA.
  • Tissue specificity
    Expressed in liver, kidney, pancreas, heart and skeletal muscle.
  • Pathway
    Lipid metabolism; fatty acid beta-oxidation.
  • Involvement in disease
    Defects in HADH are the cause of 3-alpha-hydroxyacyl-CoA dehydrogenase deficiency (HADH deficiency) [MIM:231530]. HADH deficiency is a metabolic disorder with various clinical presentations including hypoglycemia, hepatoencephalopathy, myopathy or cardiomyopathy, and in some cases sudden death.
    Defects in HADH are the cause of familial hyperinsulinemic hypoglycemia type 4 (HHF4) [MIM:609975]; also known as persistent hyperinsulinemic hypoglycemia of infancy (PHHI) or congenital hyperinsulinism. HHF is the most common cause of persistent hypoglycemia in infancy and is due to defective negative feedback regulation of insulin secretion by low glucose levels. It causes nesidioblastosis, a diffuse abnormality of the pancreas in which there is extensive, often disorganized formation of new islets. Unless early and aggressive intervention is undertaken, brain damage from recurrent episodes of hypoglycemia may occur. HHF4 should be easily recognizable by analysis of acylcarnitine species and that this disorder responds well to treatment with diazoxide. It provides the first 'experiment of nature' that links impaired fatty acid oxidation to hyperinsulinism and that provides support for the concept that a lipid signaling pathway is implicated in the control of insulin secretion.
  • Sequence similarities
    Belongs to the 3-hydroxyacyl-CoA dehydrogenase family.
  • Cellular localization
    Mitochondrion matrix.
  • Information by UniProt
  • Database links
  • Alternative names
    • 3 hydroxyacyl Coenzyme A dehydrogenase antibody
    • HAD antibody
    • HADH antibody
    • HADH1 antibody
    • HADHSC antibody
    • HADHSC, formerly antibody
    • HADSC, formerly antibody
    • HCDH antibody
    • HCDH_HUMAN antibody
    • HHF4 antibody
    • Hydroxyacyl CoA dehydrogenase antibody
    • Hydroxyacyl-coenzyme A dehydrogenase antibody
    • hydroxyacyl-coenzyme A dehydrogenase, mitochondrial antibody
    • L 3 hydroxyacyl Coenzyme A dehydrogenase short chain antibody
    • M SCHAD antibody
    • Medium and short chain L 3 hydroxyacyl coenzyme A dehydrogenase antibody
    • Medium and short-chain L-3-hydroxyacyl-coenzyme A dehydrogenase antibody
    • MGC8392 antibody
    • mitochondrial antibody
    • MSCHAD antibody
    • OTTHUMP00000162626 antibody
    • OTTHUMP00000219688 antibody
    • SCHAD antibody
    • SCHAD, formerly antibody
    • Short chain 3 hydroxyacyl CoA dehydrogenase mitochondrial antibody
    • short chain 3-hydroxyacyl-coa dehydrogenase antibody
    • Short-chain 3-hydroxyacyl-CoA dehydrogenase antibody
    see all

Images

  • MRC5 fibroblasts labeled with ab110284 at 5µg/ml and stained with Texas Red conjugated to goat anti-mouse secondary.
  • HADHSC immunocaptured from Human liver mitochondria using ab110284.
  • HL-60 cells were stained with 1 µg/ml ab110284 (blue) or an equal amount of an isotype control antibody (red) and analyzed by flow cytometry.
  • ab110284, at 1/250 dilution, staining HADHSC in formalin-fixed, paraffin-embedded Human cerebellum by Immunohistochemistry. HADHSC immunoactivity is most intense in neuronal cell bodies, most notably in the large Purkinje cells.

References

ab110284 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Question
Answer

DISCOUNT CODE: ********* Expiration date: *******I am very pleased to hear you would like to accept our offer and test ab110284 in mouse IP. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for mouse IP and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews. Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code. Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research. The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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Answer

Thank you very much for your interest in ab110284. Below are the details of how the testing discount works. If you would still like to take advantage of the discount program, please let me know and I will issue the code: To our knowledge, ab110284 has not been tested in immunoprecipitation (IP). Therefore, I can offer a discount off a future purchase if you buy ab110284 now, test it in IP and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free primary antibody. If you are interested in this offer, please follow these steps: 1. Reply to this e-mail to let me know that you would like to proceed and test ab110284 in IP. I will then send a discount code. This code must be issued before purchasing ab110284 so please wait for my reply before ordering. 2. Purchase ab110284 either by phone, fax, or online (www.abcam.com). 3. Test it in IP. 4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews. 5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any primary antibody ordered and the discount code is valid for 4 months after issue. We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab110284 turns out to be unsuitable for IP you will still receive the discount on your next purchase after your Abreview has been submitted. Please let me know if you have any questions about this offer and I would be happy to help you further. The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

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Answer

Thank you for contacting Abcam. The epitope for ab110284, the anti-HADHSC antibody has not been mapped and so unfortunately I cannot tell you where it will bind on the protein. The antibody has not been tested in mouse, but I did an alignment between the human and mouse sequences and there was a 97% homology between the two and so I would be surprised if the antibody did not work in mouse. Although since it is a mouse monoclonal, you would have to be careful about the secondary non-specifically binding to the mouse tissue. There are a few methods to overcome this, either directly conjugating the antibody with a secondary, using one of our conjugation kit. Or you could use a Fab fragment, that would help prevent non-specific binding of the secondary. If you are unsure about using the antibody in mouse and since it has not been tested, I can offer you our testing discount. If you are interested in taking advantage of this discount, please let me know and I can send you the details.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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