Anti-HDAC5 antibody [HDAC5-35] (ab50001)
Key features and details
- Mouse monoclonal [HDAC5-35] to HDAC5
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG1
Overview
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Product name
Anti-HDAC5 antibody [HDAC5-35]
See all HDAC5 primary antibodies -
Description
Mouse monoclonal [HDAC5-35] to HDAC5 -
Host species
Mouse -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Synthetic peptide corresponding to Human HDAC5 aa 4-19 conjugated to keyhole limpet haemocyanin. synthetic peptide has a C-terminal added lysine.
Sequence:PNESDGMSGREPSLEI
Database link: Q9UQL6 -
Positive control
- WB: Total cell extracts of NIH3T3 fibroblast cells.
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General notes
This product was changed from ascites to tissue culture supernatant on 05/06/2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.097% Sodium azide
Constituent: 0.0268% PBS -
Concentration information loading...
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Purity
Tissue culture supernatant -
Clonality
Monoclonal -
Clone number
HDAC5-35 -
Myeloma
NS1 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab50001 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 122 kDa.
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Notes |
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WB
Use at an assay dependent concentration. Predicted molecular weight: 122 kDa. |
Target
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Function
Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation by repressing transcription of myocyte enhancer MEF2C. During muscle differentiation, it shuttles into the cytoplasm, allowing the expression of myocyte enhancer factors. -
Tissue specificity
Ubiquitous. -
Sequence similarities
Belongs to the histone deacetylase family. HD type 2 subfamily. -
Domain
The nuclear export sequence mediates the shuttling between the nucleus and the cytoplasm. -
Post-translational
modificationsPhosphorylated by CaMK at Ser-259 and Ser-498. The phosphorylation is required for the export to the cytoplasm. Phosphorylated by the PKC kinases PKN1 and PKN2, impairing nuclear import.
Ubiquitinated. Polyubiquitination however does not lead to its degradation. -
Cellular localization
Nucleus. Cytoplasm. Shuttles between the nucleus and the cytoplasm. In muscle cells, it shuttles into the cytoplasm during myocyte differentiation. The export to cytoplasm depends on the interaction with a 14-3-3 chaperone protein and is due to its phosphorylation at Ser-259 and Ser-498 by CaMK. - Information by UniProt
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Database links
- Entrez Gene: 15184 Mouse
- SwissProt: Q9Z2V6 Mouse
- Unigene: 22665 Mouse
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Alternative names
- Antigen NY CO 9 antibody
- Antigen NY-CO-9 antibody
- HD5 antibody
see all
Images
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Lane 1 : Anti-HDAC5 antibody [HDAC5-35] (ab50001) at 2 µg/ml
Lane 2 : no primary antibody
All lanes : 3T3 cell extract (mouse fibroblasts)
Secondary
All lanes : Goat Anti-Mouse, Peroxidase conjugate
Predicted band size: 122 kDa
Observed band size: ~122 kDa why is the actual band size different from the predicted?This image was generated using the ascites version of the product.
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab50001 has been referenced in 2 publications.
- Yu XH et al. LncRNA kcnq1ot1 promotes lipid accumulation and accelerates atherosclerosis via functioning as a ceRNA through the miR-452-3p/HDAC3/ABCA1 axis. Cell Death Dis 11:1043 (2020). PubMed: 33293505
- Mitsi V et al. RGS9-2--controlled adaptations in the striatum determine the onset of action and efficacy of antidepressants in neuropathic pain states. Proc Natl Acad Sci U S A 112:E5088-97 (2015). WB ; Mouse . PubMed: 26305935