Recombinant Anti-HDLBP antibody [EPR5267] (ab109324)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5267] to HDLBP
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-HDLBP antibody [EPR5267]
See all HDLBP primary antibodies -
Description
Rabbit monoclonal [EPR5267] to HDLBP -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details
Unsuitable for: Flow Cyt or IP -
Species reactivity
Reacts with: Human
Predicted to work with: Mouse, Rat -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: 293T, Jurkat, HeLa and HepG2 cell lysates IHC-P: Human stomach tissue
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Preservative: 0.05% Sodium azide
Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR5267 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab109324 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/10000 - 1/50000. Detects a band of approximately 155 kDa (predicted molecular weight: 141 kDa).
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IHC-P |
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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Notes |
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WB
1/10000 - 1/50000. Detects a band of approximately 155 kDa (predicted molecular weight: 141 kDa). |
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
Target
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Function
Appears to play a role in cell sterol metabolism. It may function to protect cells from over-accumulation of cholesterol. -
Sequence similarities
Contains 14 KH domains. -
Cellular localization
Cytoplasm. Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 3069 Human
- Entrez Gene: 110611 Mouse
- Entrez Gene: 64474 Rat
- Omim: 142695 Human
- SwissProt: Q00341 Human
- SwissProt: Q8VDJ3 Mouse
- SwissProt: Q9Z1A6 Rat
- Unigene: 471851 Human
see all -
Alternative names
- HBP antibody
- HDL binding protein antibody
- HDL-binding protein antibody
see all
Images
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All lanes : Anti-HDLBP antibody [EPR5267] (ab109324) at 1/10000 dilution
Lane 1 : 293T cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : HepG2 cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 141 kDa
Observed band size: 155 kDa why is the actual band size different from the predicted? -
ab109324, at a 1/100 dilution, staining HDLBP in paraffin embedded Human stomach tissue by Immunohistochemistry.
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab109324 has been referenced in 2 publications.
- Zinnall U et al. HDLBP binds ER-targeted mRNAs by multivalent interactions to promote protein synthesis of transmembrane and secreted proteins. Nat Commun 13:2727 (2022). PubMed: 35585045
- Mobin MB et al. The RNA-binding protein vigilin regulates VLDL secretion through modulation of Apob mRNA translation. Nat Commun 7:12848 (2016). PubMed: 27665711