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Our Abpromise guarantee covers the use of ab22614 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-Fr||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration.|
|WB||Use a concentration of 1 - 5 µg/ml. Predicted molecular weight: 34 kDa.Can be blocked with Human HEY1 peptide (ab111660). 5% skim milk / PBS buffer, and HRP conjugated anti-Rabbit IgG should be diluted in 1/50,000 - 100,000 as second antibody. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.|
|IHC-P||Use at an assay dependent concentration.|
Paraffin embedded human lung tissue labelled with HEY1 antibody (ab22614) at 4-8µg/ml. Magnification 400X.
Alveolar cells are positively labelled (indicated with arrows).
Paraffin embeded human kidney tissue labelled with HEY1 antibody (ab22614) at 4-8µg/ml. Magnification 400X.
Epithelial cells of renal tubules are positively labelled (indicated with arrows).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"