Anti-HGS antibody (ab72053)
Key features and details
- Rabbit polyclonal to HGS
- Suitable for: WB, IP
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-HGS antibody
See all HGS primary antibodies -
Description
Rabbit polyclonal to HGS -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Cow -
Immunogen
Synthetic peptide within Human HGS aa 700 to the C-terminus (C terminal). The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: O14964 -
Positive control
- WB: HeLa, Renca, CT26.WT and 4T1 whole cell lysate. IP: HeLa whole cell lysate.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
ab72053 was affinity purified using an epitope specific to HGS immobilized on solid support. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab72053 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/2000 - 1/10000. Detects a band of approximately 110 kDa (predicted molecular weight: 86 kDa).
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IP |
Use at 2-10 µg/mg of lysate.
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Notes |
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WB
1/2000 - 1/10000. Detects a band of approximately 110 kDa (predicted molecular weight: 86 kDa). |
IP
Use at 2-10 µg/mg of lysate. |
Target
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Function
Involved in intracellular signal transduction mediated by cytokines and growth factors. When associated with STAM, it suppresses DNA signaling upon stimulation by IL-2 and GM-CSF. Could be a direct effector of PI3-kinase in vesicular pathway via early endosomes and may regulate trafficking to early and late endosomes by recruiting clathrin. May concentrate ubiquitinated receptors within clathrin-coated regions. Involved in down-regulation of receptor tyrosine kinase via multivesicular body (MVBs) when complexed with STAM (ESCRT-0 complex). The ESCRT-0 complex binds ubiquitin and acts as sorting machinery that recognizes ubiquitinated receptors and transfers them to further sequential lysosomal sorting/trafficking processes. May contribute to the efficient recruitment of SMADs to the activin receptor complex. Involved in receptor recycling via its association with the CART complex, a multiprotein complex required for efficient transferrin receptor recycling but not for EGFR degradation. -
Tissue specificity
Ubiquitous expression in adult and fetal tissues with higher expression in testis and peripheral blood leukocytes. -
Sequence similarities
Contains 1 FYVE-type zinc finger.
Contains 1 UIM (ubiquitin-interacting motif) repeat.
Contains 1 VHS domain. -
Domain
Has a double-sided UIM that can bind 2 ubiquitin molecules, one on each side of the helix.
The FYVE-type zinc finger domain mediates interactions with phosphatidylinositol 3-phosphate in membranes of early endosomes and penetrates bilayers. The FYVE domain insertion into PtdIns(3)P-enriched membranes is substantially increased in acidic conditions. -
Post-translational
modificationsPhosphorylated on Tyr-334. A minor site of phosphorylation on Tyr-329 is detected (By similarity). Phosphorylation occurs in response to EGF, IL-2, GM-CSF and HGF.
Ubiquitinated by ITCH. -
Cellular localization
Cytoplasm. Early endosome membrane. Endosome > multivesicular body membrane. - Information by UniProt
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Database links
- Entrez Gene: 511582 Cow
- Entrez Gene: 9146 Human
- Omim: 604375 Human
- SwissProt: Q0V8S0 Cow
- SwissProt: O14964 Human
- Unigene: 514590 Human
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Alternative names
- Hepatocyte growth factor regulated tyrosine kinase substrate antibody
- Hepatocyte growth factor-regulated tyrosine kinase substrate antibody
- HGF-regulated tyrosine kinase substrate antibody
see all
Images
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All lanes : Anti-HGS antibody (ab72053) at 0.1 µg/ml
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Predicted band size: 86 kDa
Exposure time: 10 secondsLysates prepared using NETN lysis buffer.
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Detection of human HGS by western blot of immunoprecipitates.
Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer.
Antibodies: ab72053 used for IP at 6 µg per reaction, lanes 1 and 2, lane 3 is control IgG. For blotting immunoprecipitated HGS, ab72053 was used at 0.4 µg/ml. -
All lanes : Anti-HGS antibody (ab72053) at 0.1 µg/ml
Lane 1 : Renca (Mouse renal adenocarcinoma cell line) whole cell lysate
Lane 2 : CT26.WT (Mouse renal colorectal carcinoma cell line) whole cell lysate
Lane 3 : 4T1 (Mouse mammary gland cell line) whole cell lysate
Lysates/proteins at 15 µg per lane.
Predicted band size: 86 kDa
Exposure time: 3 minutesLysates prepared using NETN lysis buffer.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (8)
ab72053 has been referenced in 8 publications.
- Suárez H et al. CD9 inhibition reveals a functional connection of extracellular vesicle secretion with mitophagy in melanoma cells. J Extracell Vesicles 10:e12082 (2021). PubMed: 34012515
- Rojas JM et al. Inhibition of the IFN Response by Bluetongue Virus: The Story So Far. Front Microbiol 12:692069 (2021). PubMed: 34168637
- Avia M et al. Virus-induced autophagic degradation of STAT2 as a mechanism for interferon signaling blockade. EMBO Rep 20:e48766 (2019). PubMed: 31603272
- Torralba D et al. Priming of dendritic cells by DNA-containing extracellular vesicles from activated T cells through antigen-driven contacts. Nat Commun 9:2658 (2018). PubMed: 29985392
- Frattini V et al. A metabolic function of FGFR3-TACC3 gene fusions in cancer. Nature 553:222-227 (2018). PubMed: 29323298
- Gómez-Escudero J et al. E-cadherin cleavage by MT2-MMP regulates apical junctional signaling and epithelial homeostasis in the intestine. J Cell Sci 130:4013-4027 (2017). PubMed: 29061881
- Villarroya-Beltri C et al. ISGylation controls exosome secretion by promoting lysosomal degradation of MVB proteins. Nat Commun 7:13588 (2016). ICC/IF . PubMed: 27882925
- Wang Y et al. Molecular characterization of proprotein convertase subtilisin/kexin type 9-mediated degradation of the LDLR. J Lipid Res 53:1932-43 (2012). PubMed: 22764087