Anti-HIV1 p24 antibody [24-2] (ab85536)
Key features and details
- Mouse monoclonal [24-2] to HIV1 p24
- Suitable for: WB, IP, ELISA, ICC/IF
- Reacts with: Species independent
- Isotype: IgG1
Overview
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Product name
Anti-HIV1 p24 antibody [24-2]
See all HIV1 p24 primary antibodies -
Description
Mouse monoclonal [24-2] to HIV1 p24 -
Host species
Mouse -
Tested applications
Suitable for: WB, IP, ELISA, ICC/IFmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Recombinant HIV1 p24 protein (clade B, HXB-3 isolate) with an N-terminal His tag, expressed in bacteria
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Constituents: 0.05% Glycerol, 0.00061% Tris, 2.94% Sodium citrate -
Concentration information loading... -
Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
24-2 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab85536 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use a concentration of 0.3 - 0.6 µg/ml. Predicted molecular weight: 28 kDa.
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| IP |
Use a concentration of 5 - 10 µg/ml.
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| ELISA |
Use a concentration of 0.6 - 1.2 µg/ml.
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| ICC/IF |
Use a concentration of 1.2 - 2.5 µg/ml.
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| Notes |
|---|
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WB
Use a concentration of 0.3 - 0.6 µg/ml. Predicted molecular weight: 28 kDa. |
|
IP
Use a concentration of 5 - 10 µg/ml. |
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ELISA
Use a concentration of 0.6 - 1.2 µg/ml. |
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ICC/IF
Use a concentration of 1.2 - 2.5 µg/ml. |
Target
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Relevance
HIV1 performs highly complex orchestrated tasks during the assembly, budding, maturation and infection stages of the viral replication cycle. During viral assembly, the proteins form membrane associations and self-associations that ultimately result in budding of an immature virion from the infected cell. Gag precursors also function during viral assembly to selectively bind and package two plus strands of genomic RNA. Capsid protein p24 probably forms the conical core of the virus that encapsulates the genomic RNA-nucleocapsid complex. -
Cellular localization
Membrane -
Alternative names
- CA antibody
- Capsid protein p24 antibody
- HIV-1 Gag p24 antibody
see all
Protocols
Datasheets and documents
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Datasheet download
References (0)
ab85536 has not yet been referenced specifically in any publications.
