The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Use a concentration of 0.1 µg/ml. Predicted molecular weight: 41 kDa.
Use a concentration of 0.1 µg/ml.
Use at an assay dependent concentration.
Is unsuitable for IP.
Human papillomaviruses (HPV) are small DNA viruses which infect epithelia of the skin and mucosa. Over 90 types have been identified and they mostly cause a variety of benign lesions such as warts and verrucae. However, some subtypes, notably types 16 and 18, 31 and 33, have been confirmed as agents which cause cervical cancer. Human Papillomavirus (HPV) E2 proteins are the major viral regulators of transcription and replication during the viral life cycle.
Western blot - Anti-HPV18 E2 antibody [2E7] (ab100969)
All lanes : Anti-HPV18 E2 antibody [2E7] (ab100969) at 1/100 dilution
Lane 1 : lysates of U20S cells transfected with plasmid expressing HPV11 E2 protein Lane 2 : lysates of plain U20S cells Lane 3 : lysates of plain HeLa cells Lane 4 : lysates of U20S transfected with plasmid expressing HPV11 E2 protein Lane 5 : marker
Cruz-Gregorio A et al. Human Papillomavirus Types 16 and 18 Early-expressed Proteins Differentially Modulate the Cellular Redox State and DNA Damage. Int J Biol Sci14:21-35 (2018).
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