Overview

  • Product name

    Anti-HSD17B1 antibody [EP1681Y]
    See all HSD17B1 primary antibodies
  • Description

    Rabbit monoclonal [EP1681Y] to HSD17B1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, Flow Cyt, ICC/IFmore details
    Unsuitable for: IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to residues on the C terminus of Human HSD17B1 (UniProt P14061).

  • Positive control

    • Human placenta lysate and Human placenta tissue
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab134193 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/2000. Predicted molecular weight: 35 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cyt 1/40.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF 1/50.
  • Application notes
    Is unsuitable for IP.
  • Target

    • Function

      Favors the reduction of estrogens and androgens. Also has 20-alpha-HSD activity. Uses preferentially NADH.
    • Pathway

      Steroid biosynthesis; estrogen biosynthesis.
    • Sequence similarities

      Belongs to the short-chain dehydrogenases/reductases (SDR) family.
    • Cellular localization

      Cytoplasm.
    • Information by UniProt
    • Database links

    • Alternative names

      • 17 beta HSD 1 antibody
      • 17 beta hydroxysteroid dehydrogenase type 1 antibody
      • 17-beta-HSD 1 antibody
      • 17-beta-hydroxysteroid dehydrogenase type 1 antibody
      • 20 alpha-hydroxysteroid dehydrogenase antibody
      • 20-alpha-HSD antibody
      • DHB1_HUMAN antibody
      • E17KSR antibody
      • E2DH antibody
      • EDH17B1 antibody
      • EDH17B2 antibody
      • EDHB17 antibody
      • Estradiol 17 beta dehydrogenase 1 antibody
      • Estradiol 17-beta-dehydrogenase 1 antibody
      • HSD17 antibody
      • HSD17B1 antibody
      • Hydroxysteroid (17 beta) dehydrogenase 1 antibody
      • MGC13814 antibody
      • Placental 17 beta hydroxysteroid dehydrogenase antibody
      • Placental 17-beta-hydroxysteroid dehydrogenase antibody
      • SDR28C1 antibody
      • Short chain dehydrogenase/reductase family 28CE,member 1 antibody
      see all

    Images

    • Anti-HSD17B1 antibody [EP1681Y] (ab134193) at 1/500 dilution + Human placenta lysate at 10 µg

      Secondary
      HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 35 kDa

    • Immunohistochemical analysis of paraffin-embedded Human placenta tissue labelling HSD17B1 with ab134193 at 1/100 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    References

    ab134193 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab134193.
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    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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