Anti-HSPC142 antibody (ab167202)
Key features and details
- Mouse polyclonal to HSPC142
- Suitable for: WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-HSPC142 antibody
See all HSPC142 primary antibodies -
Description
Mouse polyclonal to HSPC142 -
Host species
Mouse -
Tested applications
Suitable for: WB, ICC/IFmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Rat, Cow, Common marmoset, Orangutan -
Immunogen
Full length protein, corresponding to amino acids 1-329 of Human HSPC142 (NP_001028721.1).
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Positive control
- Hela cells; Lysate of 293T cells transfected with HSPC142.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. -
Storage buffer
pH: 7.4
Constituent: 99% PBS -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab167202 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
Use a concentration of 1 µg/ml. Predicted molecular weight: 37 kDa.
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ICC/IF |
Use a concentration of 10 µg/ml.
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Notes |
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WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 37 kDa. |
ICC/IF
Use a concentration of 10 µg/ml. |
Target
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Function
Component of the BRCA1-A complex, a complex that specifically recognizes 'Lys-63'-linked ubiquitinated histones H2A and H2AX at DNA lesions sites, leading to target the BRCA1-BARD1 heterodimer to sites of DNA damage at double-strand breaks (DSBs). The BRCA1-A complex also possesses deubiquitinase activity that specifically removes 'Lys-63'-linked ubiquitin on histones H2A and H2AX. In the BRCA1-A complex, it is required for the complex integrity and its localization at DSBs. Probably also plays a role as a component of the BRISC complex, a multiprotein complex that specifically cleaves 'Lys-63'-linked ubiquitin. In these 2 complexes, it is probably required to maintain the stability of BRE/BRCC45 and help the 'Lys-63'-linked deubiquitinase activity mediated by BRCC3/BRCC36. component. -
Sequence similarities
Belongs to the BABAM1 family. -
Domain
The VWFA-like region is similar to the VWFA domain. Its presence reveals similarities between the structure of the 19S proteasome and the BRCA1-A complexes. -
Cellular localization
Cytoplasm. Nucleus. Localizes at sites of DNA damage at double-strand breaks. - Information by UniProt
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Database links
- Entrez Gene: 29086 Human
- Entrez Gene: 290631 Rat
- Omim: 612766 Human
- SwissProt: Q9NWV8 Human
- SwissProt: Q5XIJ6 Rat
- Unigene: 190722 Human
- Unigene: 74107 Rat
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Alternative names
- BABA1_HUMAN antibody
- babam1 antibody
- BRCA1 A complex subunit MERIT40 antibody
see all
Images
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All lanes : Anti-HSPC142 antibody (ab167202) at 1 µg/ml
Lane 1 : Lysate of 293T cells transfected with HSPC142
Lane 2 : Lysate of 293T cells (non-transfected)
Lysates/proteins at 15 µl per lane.
Secondary
All lanes : Goat anti-mouse HRP conjugated antibody at 1/2500 dilution
Predicted band size: 37 kDa -
Immunofluorescent analysis of HeLa cells labeling HSPC142 with ab167202 at 10 ug/ml.
Protocols
Datasheets and documents
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Datasheet download
References (0)
ab167202 has not yet been referenced specifically in any publications.