Anti-Hemoglobin antibody [Haem1] (ab401)
Key features and details
- Mouse monoclonal [Haem1] to Hemoglobin
- Suitable for: ELISA, RIA
- Reacts with: Human
- Isotype: IgG1
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-Hemoglobin antibody [Haem1]
See all Hemoglobin primary antibodies -
Description
Mouse monoclonal [Haem1] to Hemoglobin -
Host species
Mouse -
Specificity
hHb - 100% hAlbumin - <0.01% hHaptoglobin - 0.4% hMyoglobin - <0.03% hTransferrin - <0.01% Cross-reactivity values have been calculated on weight/weight basis. They can vary because of the presence of antigen or related impurities in protein preparations used for cross reactivity assays. Affinity constant: 1x1010 l/mol human haemoglobin. -
Tested applications
Suitable for: ELISA, RIAmore details -
Species reactivity
Reacts with: Human -
Immunogen
Full length native protein (purified) (Human).
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 6.00
Preservative: 0.095% Sodium azide
Constituents: 0.335% PBS, 1.0878% Sodium citrate -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
Haem1 -
Myeloma
unknown -
Isotype
IgG1 -
Light chain type
unknown -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab401 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ELISA |
Use at an assay dependent concentration.
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RIA |
Use at an assay dependent concentration.
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Notes |
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ELISA
Use at an assay dependent concentration. |
RIA
Use at an assay dependent concentration. |
Target
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Function
Involved in oxygen transport from the lung to the various peripheral tissues. -
Tissue specificity
Red blood cells. -
Involvement in disease
Defects in HBA1/HBA2 may be a cause of Heinz body anemias (HEIBAN) [MIM:140700]. This is a form of non-spherocytic hemolytic anemia of Dacie type 1. After splenectomy, which has little benefit, basophilic inclusions called Heinz bodies are demonstrable in the erythrocytes. Before splenectomy, diffuse or punctate basophilia may be evident. Most of these cases are probably instances of hemoglobinopathy. The hemoglobin demonstrates heat lability. Heinz bodies are observed also with the Ivemark syndrome (asplenia with cardiovascular anomalies) and with glutathione peroxidase deficiency.
Defects in HBA1/HBA2 are the cause of alpha-thalassemia (A-THAL) [MIM:604131]. The thalassemias are the most common monogenic diseases and occur mostly in Mediterranean and Southeast Asian populations. The hallmark of alpha-thalassemia is an imbalance in globin-chain production in the adult HbA molecule. The level of alpha chain production can range from none to very nearly normal levels. Deletion of both copies of each of the two alpha-globin genes causes alpha(0)-thalassemia, also known as homozygous alpha thalassemia. Due to the complete absence of alpha chains, the predominant fetal hemoglobin is a tetramer of gamma-chains (Bart hemoglobin) that has essentially no oxygen carrying capacity. This causes oxygen starvation in the fetal tissues leading to prenatal lethality or early neonatal death. The loss of three alpha genes results in high levels of a tetramer of four beta chains (hemoglobin H), causing a severe and life-threatening anemia known as hemoglobin H disease. Untreated, most patients die in childhood or early adolescence. The loss of two alpha genes results in mild alpha-thalassemia, also known as heterozygous alpha-thalassemia. Affected individuals have small red cells and a mild anemia (microcytosis). If three of the four alpha-globin genes are functional, individuals are completely asymptomatic. Some rare forms of alpha-thalassemia are due to point mutations (non-deletional alpha-thalassemia). The thalassemic phenotype is due to unstable globin alpha chains that are rapidly catabolized prior to formation of the alpha-beta heterotetramers.
Note=Alpha(0)-thalassemia is associated with non-immune hydrops fetalis, a generalized edema of the fetus with fluid accumulation in the body cavities due to non-immune causes. Non-immune hydrops fetalis is not a diagnosis in itself but a symptom, a feature of many genetic disorders, and the end-stage of a wide variety of disorders. -
Sequence similarities
Belongs to the globin family. -
Post-translational
modificationsThe initiator Met is not cleaved in variant Thionville and is acetylated. - Information by UniProt
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Database links
- Entrez Gene: 3039 Human
- Entrez Gene: 3040 Human
- Entrez Gene: 3043 Human
- Entrez Gene: 3047 Human
- Omim: 141800 Human
- Omim: 141900 Human
- Omim: 142200 Human
- SwissProt: P68871 Human
see all -
Alternative names
- 3-prime alpha-globin gene antibody
- A gamma globin antibody
- Alpha 1 globin antibody
see all
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab401 has been referenced in 2 publications.
- Nakazawa H et al. Coenzyme Q10 protects against burn-induced mitochondrial dysfunction and impaired insulin signaling in mouse skeletal muscle. FEBS Open Bio 9:348-363 (2019). PubMed: 30761259
- Liu DN et al. Superior mesenteric artery margin in pancreaticoduodenectomy for pancreatic adenocarcinoma. Oncotarget 8:7766-7776 (2017). PubMed: 27999192