Anti-Hepatitis C Virus NS3 antibody [H23] (ab13830)
Key features and details
- Mouse monoclonal [H23] to Hepatitis C Virus NS3
- Suitable for: ELISA, WB, IHC-P
- Reacts with: Hepatitis C virus
- Isotype: IgG1
Overview
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Product name
Anti-Hepatitis C Virus NS3 antibody [H23]
See all Hepatitis C Virus NS3 primary antibodies -
Description
Mouse monoclonal [H23] to Hepatitis C Virus NS3 -
Host species
Mouse -
Specificity
Hepatitis C Virus antigen NS3 helicase domain. Reacts very well with JFH-1 strain (genotype 2a) -
Tested applications
Suitable for: ELISA, WB, IHC-Pmore details -
Species reactivity
Reacts with: Hepatitis C virus -
Immunogen
Recombinant full length protein corresponding to Hepatitis C Virus NS3.
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Epitope
Helicase domain -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.1% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein G purified -
Clonality
Monoclonal -
Clone number
H23 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab13830 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ELISA |
Use at an assay dependent concentration.
|
|
WB |
1/1000.
|
|
IHC-P |
1/50.
Tested using paraffin-embedded liver sections from HCV patients. |
Notes |
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ELISA
Use at an assay dependent concentration. |
WB
1/1000. |
IHC-P
1/50. Tested using paraffin-embedded liver sections from HCV patients. |
Target
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Relevance
HCV is a positive, single-stranded RNA virus in the Flaviviridae family. The genome is approximately 10,000 nucleotides and encodes a single polyprotein of about 3,000 amino acids. The polyprotein is processed by host cell and viral proteases into three major structural proteins including NS3, and several non-structural proteins necessary for viral replication. The NS3 part of the polyprotein displays three enzymatic activities: serine protease, NTPase and RNA helicase. The NS3 serine proteinase (NS3P) is a non-structural hepatitis C protein responsible for proteolytic processing of other non-structural proteins; because of this, it is also the most extensively studied protein of the Hepatitis C genome. It is responsible for proteolytic processing of the entire downstream region of the HC polyprotein, catalyzing cleavage at the NS3/NS4a, NS4a/NS4b, NS4b/NS5a, and NS5a/NS5b sites to release the mature NS3, NS4a, NS4b, NS5a, and NS5b proteins.For proper function, NS3 requires NS4a as a cofactor, but, interestingly enough, NS3 also cleaves the NS4a protein. The molecular weight of the monomer NS3P is 70 kDa. -
Cellular localization
Endoplasmic reticulum membrane -
Database links
- SwissProt: P26663 Hepatitis C virus
- SwissProt: P26664 Hepatitis C virus
- SwissProt: Q81258 Hepatitis C virus
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Alternative names
- Hepatitis C Virus NS3 antibody
- NS3 antibody
- NS3P antibody
see all
Images
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ab13830 used in IHC at 1/50 dilution. Cell line used is Huh7 human hepatoma.
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The negative control for above picture with Huh7 cells.
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Representative Western Blot detecting Hepatitis C Virus NS3 with ab13830.
SL209-biotin was immobilized on streptavidin agarose beads and incubated with lysates of Huh-7.5 cells infected with HCV J6/JFH-1. Retained proteins were examined by western blot.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (36)
ab13830 has been referenced in 36 publications.
- Li H et al. Identification and Verification of Ubiquitin D as a Gene Associated with Hepatitis C Virus-Induced Hepatocellular Carcinoma. Intervirology 65:195-205 (2022). PubMed: 35728518
- Widyawaruyanti A et al. Alkaloid and benzopyran compounds of Melicope latifolia fruit exhibit anti-hepatitis C virus activities. BMC Complement Med Ther 21:27 (2021). PubMed: 33435968
- Zou LL et al. TGF-ß isoforms inhibit hepatitis C virus propagation in transforming growth factor beta/SMAD protein signalling pathway dependent and independent manners. J Cell Mol Med 25:3498-3510 (2021). PubMed: 33682288
- Li J et al. Reticulon-3 modulates the incorporation of replication competent hepatitis C virus molecules for release inside infectious exosomes. PLoS One 15:e0239153 (2020). PubMed: 32941510
- Ma YY et al. IL28A protein homotetramer structure is required for autolysosomal degradation of HCV-NS5A in vitro. Cell Death Dis 11:200 (2020). PubMed: 32205851