High Sensitivity Carbohydrate Assay Kit (Colorimetric) (ab284543)
Key features and details
- Assay type: Quantitative
- Detection method: Colorimetric
- Platform: Microplate (12 x 8 well strips)
- Sample type: Adherent cells, Food samples, Other biological fluids, Suspension cells, Tissue
Overview
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Product name
High Sensitivity Carbohydrate Assay Kit (Colorimetric)
See all Carbohydrate kits -
Detection method
Colorimetric -
Sample type
Other biological fluids, Tissue, Adherent cells, Suspension cells, Food samples -
Assay type
Quantitative -
Range
0 µg - 20 µg -
Assay duration
Multiple steps standard assay -
Product overview
High Sensitivity Carbohydrate Assay Kit (Colorimetric) (ab284543) (K2049) is a simple, sensitive, robust assay for the quantification of carbohydrates even when present in low concentration. The assay can be used for the quantitative determination of any carbohydrates that contain neutral sugars and uronic acids. The reaction is a modification of phenol-sulfuric acid method.
In this assay, carbohydrates are hydrolyzed and converted to hydroxyfurfural and furfural, which then react with a highly sensitive developer to form a colored complex, detected spectrophotometrically at 500 nm.
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Notes
This product is manufactured by BioVision, an Abcam company and was previously called K2049 High Sensitivity Carbohydrate Assay Kit (Colorimetric). K2049-100 is the same size as the 100 test size of ab284543.
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Platform
Microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components Identifier 100 tests Carbohydrate Developer Amber 1 x 1.5ml Standard (D-Glucose, 2 mg/ml) Yellow 1 x 0.2ml
Images
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Total Polysaccharide content in Pneumococcal Polysaccharide Powder Type 4 (US Type 4)
Total Polysaccharide content in Pneumococcal Polysaccharide Powder Type 4 (US Type 4), calculated total carbohydrate concentration (glucose equivalent) is 0.47 ± 0.02 μg/μL. The assay was performed according to the kit assay protocol.
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Absorbance of MonosaccharidesAbsorbance of monosaccharides upon reacting with High Sensitivity (HS) Carbohydrate Developer and Phenol. Assays were performed following the kit protocol.
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D-Glucose Standard CurveD-Glucose Standard Curve. Assays were performed following the kit protocol.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab284543 has not yet been referenced specifically in any publications.