Anti-Histone H4 antibody (ab16483)
Key features and details
- Rabbit polyclonal to Histone H4
- Suitable for: WB
- Reacts with: Cow, Human, Recombinant fragment
- Isotype: IgG
Overview
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Product name
Anti-Histone H4 antibody
See all Histone H4 primary antibodies -
Description
Rabbit polyclonal to Histone H4 -
Host species
Rabbit -
Specificity
We recommend ab16483 as a suitable alternative to ab10156 as it was made using the same immunogen. -
Tested applications
Suitable for: WBmore details -
Species reactivity
Reacts with: Cow, Human, Recombinant fragment
Predicted to work with: Saccharomyces cerevisiae -
Immunogen
Recombinant full length protein corresponding to Saccharomyces cerevisiae Histone H4 aa 1 to the C-terminus.
Database link: P02309 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab16483 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB | (1) |
Use a concentration of 1 µg/ml. Detects a band of approximately 14 kDa (predicted molecular weight: 11 kDa).
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Notes |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 14 kDa (predicted molecular weight: 11 kDa). |
Target
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Function
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. -
Sequence similarities
Belongs to the histone H4 family. -
Post-translational
modificationsAcetylation at Lys-6 (H4K5ac), Lys-9 (H4K8ac), Lys-13 (H4K12ac) and Lys-17 (H4K16ac) occurs in coding regions of the genome but not in heterochromatin.
Citrullination at Arg-4 (H4R3ci) by PADI4 impairs methylation.
Monomethylation and asymmetric dimethylation at Arg-4 (H4R3me1 and H4R3me2a, respectively) by PRMT1 favors acetylation at Lys-9 (H4K8ac) and Lys-13 (H4K12ac). Demethylation is performed by JMJD6. Symmetric dimethylation on Arg-4 (H4R3me2s) by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage.
Monomethylated, dimethylated or trimethylated at Lys-21 (H4K20me1, H4K20me2, H4K20me3). Monomethylation is performed by SET8. Trimethylation is performed by SUV420H1 and SUV420H2 and induces gene silencing.
Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins. Monoubiquitinated at Lys-92 of histone H4 (H4K91ub1) in response to DNA damage. The exact role of H4K91ub1 in DNA damage response is still unclear but it may function as a licensing signal for additional histone H4 post-translational modifications such as H4 Lys-21 methylation (H4K20me).
Sumoylated, which is associated with transcriptional repression. -
Cellular localization
Nucleus. Chromosome. - Information by UniProt
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Database links
- Entrez Gene: 121504 Human
- Entrez Gene: 554313 Human
- Entrez Gene: 8294 Human
- Entrez Gene: 8359 Human
- Entrez Gene: 8360 Human
- Entrez Gene: 8361 Human
- Entrez Gene: 8362 Human
- Entrez Gene: 8363 Human
see all -
Alternative names
- dJ160A22.1 antibody
- dJ160A22.2 antibody
- dJ221C16.1 antibody
see all
Images
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All lanes : Anti-Histone H4 antibody (ab16483) at 1 µg/ml
Lane 1 : Calf Thymus Histone lysate at 0.5 µg
Lane 2 : HeLa Histone Lysate at 2.5 µg
Lane 3 : Recombinant Histone H4 at 1 µg
Secondary
All lanes : Alexa Fluor Goat polyclonal to Rabbit IgG at 1/10000 dilution
Predicted band size: 11 kDa
Observed band size: 14 kDa why is the actual band size different from the predicted?ab16483 detects a band of approximately 14 kDa in Calf thymus histone prep, HeLa histone prep and recombinant Histone H4. This is slightly larger than the predicted molecular weight according to Swissprot (11 kDa), however we are confident that ab16483 is recognising Histone H4.
Datasheets and documents
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SDS download
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Datasheet download
References (14)
ab16483 has been referenced in 14 publications.
- Bhattacharjee P et al. Epigenetic regulations in alternative telomere lengthening: Understanding the mechanistic insight in arsenic-induced skin cancer patients. Sci Total Environ 704:135388 (2020). PubMed: 31837846
- Koziol K et al. Changes in ?H2AX and H4K16ac levels are involved in the biochemical response to a competitive soccer match in adolescent players. Sci Rep 10:14481 (2020). PubMed: 32879387
- Selvarajah B et al. mTORC1 amplifies the ATF4-dependent de novo serine-glycine pathway to supply glycine during TGF-ß1-induced collagen biosynthesis. Sci Signal 12:N/A (2019). PubMed: 31113850
- Vadla R & Haldar D Mammalian target of rapamycin complex 2 (mTORC2) controls glycolytic gene expression by regulating Histone H3 Lysine 56 acetylation. Cell Cycle 17:110-123 (2018). PubMed: 29143563
- Khan MA et al. Regulating NETosis: Increasing pH Promotes NADPH Oxidase-Dependent NETosis. Front Med (Lausanne) 5:19 (2018). PubMed: 29487850