Anti-Histone H4 (symmetric di methyl R3) antibody (ab231658)
Key features and details
- Rabbit polyclonal to Histone H4 (symmetric di methyl R3)
- Suitable for: ICC/IF, Dot blot, WB
- Reacts with: Human, Recombinant fragment
Overview
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Product name
Anti-Histone H4 (symmetric di methyl R3) antibody
See all Histone H4 primary antibodies -
Description
Rabbit polyclonal to Histone H4 (symmetric di methyl R3) -
Host species
Rabbit -
Specificity
ab231658 also recognizes H2AR3me2(sym). -
Tested applications
Suitable for: ICC/IF, Dot blot, WBmore details -
Species reactivity
Reacts with: Human, Recombinant fragment -
Immunogen
Synthetic peptide corresponding to Human Histone H4 (symmetric di methyl R3) conjugated to keyhole limpet haemocyanin.
Database link: P62805 -
Positive control
- ICC/IF: HeLa cells. WB: Whole cell and histone extracts from HeLa cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservatives: 0.05% Sodium azide, 0.05% Proclin 300
Constituent: PBS -
Concentration information loading...
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Purity
Affinity purified -
Clonality
Polyclonal
Associated products
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Compatible Secondaries
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab231658 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC/IF |
1/500.
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|
Dot blot |
1/5000.
|
|
WB |
1/1000.
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Notes |
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ICC/IF
1/500. |
Dot blot
1/5000. |
WB
1/1000. |
Target
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Function
Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. -
Sequence similarities
Belongs to the histone H4 family. -
Post-translational
modificationsAcetylation at Lys-6 (H4K5ac), Lys-9 (H4K8ac), Lys-13 (H4K12ac) and Lys-17 (H4K16ac) occurs in coding regions of the genome but not in heterochromatin.
Citrullination at Arg-4 (H4R3ci) by PADI4 impairs methylation.
Monomethylation and asymmetric dimethylation at Arg-4 (H4R3me1 and H4R3me2a, respectively) by PRMT1 favors acetylation at Lys-9 (H4K8ac) and Lys-13 (H4K12ac). Demethylation is performed by JMJD6. Symmetric dimethylation on Arg-4 (H4R3me2s) by the PRDM1/PRMT5 complex may play a crucial role in the germ-cell lineage.
Monomethylated, dimethylated or trimethylated at Lys-21 (H4K20me1, H4K20me2, H4K20me3). Monomethylation is performed by SET8. Trimethylation is performed by SUV420H1 and SUV420H2 and induces gene silencing.
Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins. Monoubiquitinated at Lys-92 of histone H4 (H4K91ub1) in response to DNA damage. The exact role of H4K91ub1 in DNA damage response is still unclear but it may function as a licensing signal for additional histone H4 post-translational modifications such as H4 Lys-21 methylation (H4K20me).
Sumoylated, which is associated with transcriptional repression. -
Cellular localization
Nucleus. Chromosome. - Information by UniProt
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Database links
- Entrez Gene: 121504 Human
- Entrez Gene: 554313 Human
- Entrez Gene: 8294 Human
- Entrez Gene: 8359 Human
- Entrez Gene: 8360 Human
- Entrez Gene: 8361 Human
- Entrez Gene: 8362 Human
- Entrez Gene: 8363 Human
see all -
Alternative names
- dJ160A22.1 antibody
- dJ160A22.2 antibody
- dJ221C16.1 antibody
see all
Images
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All lanes : Anti-Histone H4 (symmetric di methyl R3) antibody (ab231658) at 1/1000 dilution
Lane 1 : Whole cell HeLa (Human epithelial cell line from cervix adenocarcinoma) extracts at 25 µg
Lane 2 : Histone extracts from HeLa (Human epithelial cell line from cervix adenocarcinoma) cells at 15 µg
Lane 3 : Recombinant histone H2A at 1 µg
Lane 4 : Recombinant histone H2B at 1 µg
Lane 5 : Recombinant histone H3 at 1 µg
Lane 6 : Recombinant histone H4 at 1 µgDilution buffer: TBS-Tween containing 5% skimmed milk.
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HeLa (Human epithelial cell line from cervix adenocarcinoma) cells stained for Histone H4 (symmetric di methyl R3) using ab231658 at a dilution of 1/500 in ICC/IF.
Cells were fxed with 4% formaldehyde for 10 minutes and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. Secondary used is an Alexa Fluor®488-conjugated anti-Rabbit IgG. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.
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Dot Blot analysis was performed with peptides containing other histone arginine methylations and the unmodifed H4R3. One hundred to 0.2 pmol of the respective peptides were spotted on a membrane. ab231658 was used at a dilution of 1:5,000.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab231658 has not yet been referenced specifically in any publications.