Anti-Hsp90 alpha antibody [5G5] (ab128483)
Key features and details
- Mouse monoclonal [5G5] to Hsp90 alpha
- Suitable for: WB, ELISA, IHC-P, Flow Cyt
- Reacts with: Mouse, Rat, Human, African green monkey
- Isotype: IgG1
Overview
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Product name
Anti-Hsp90 alpha antibody [5G5]
See all Hsp90 alpha primary antibodies -
Description
Mouse monoclonal [5G5] to Hsp90 alpha -
Host species
Mouse -
Tested applications
Suitable for: WB, ELISA, IHC-P, Flow Cytmore details -
Species reactivity
Reacts with: Mouse, Rat, Human, African green monkey -
Immunogen
Purified recombinant fragment of Human Hsp90 alpha expressed in E. coli.
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Positive control
- Human Hsp90 alpha (amino acids 275-484) recombinant protein; NIH 3T3, HeLa, HCT116, HL60, SK-Br-3, C6 and C0S7 cell lysates
Flow Cyt: HeLa cells
IHC-P: Lung cancer tissue
- Human Hsp90 alpha (amino acids 275-484) recombinant protein; NIH 3T3, HeLa, HCT116, HL60, SK-Br-3, C6 and C0S7 cell lysates
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.05% Sodium azide
Constituent: 99% PBS
Contains 0.5% protein stabilizer. -
Concentration information loading...
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Purity
Protein G purified -
Purification notes
Purified from tissue culture supernatant. -
Clonality
Monoclonal -
Clone number
5G5 -
Isotype
IgG1 -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab128483 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/500 - 1/2000. Predicted molecular weight: 85 kDa.
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ELISA |
1/10000.
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IHC-P |
1/200 - 1/1000.
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Flow Cyt |
1/200 - 1/400.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Notes |
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WB
1/500 - 1/2000. Predicted molecular weight: 85 kDa. |
ELISA
1/10000. |
IHC-P
1/200 - 1/1000. |
Flow Cyt
1/200 - 1/400. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
Target
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Function
Molecular chaperone. Has ATPase activity. -
Sequence similarities
Belongs to the heat shock protein 90 family. -
Post-translational
modificationsISGylated. -
Cellular localization
Cytoplasm. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. - Information by UniProt
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Database links
- Entrez Gene: 3320 Human
- Entrez Gene: 15519 Mouse
- Entrez Gene: 299331 Rat
- Omim: 140571 Human
- SwissProt: P07900 Human
- SwissProt: P07901 Mouse
- SwissProt: P82995 Rat
- Unigene: 525600 Human
see all -
Alternative names
- EL52 antibody
- epididymis luminal secretory protein 52 antibody
- Heat shock 86 kDa antibody
see all
Images
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Anti-Hsp90 alpha antibody [5G5] (ab128483) at 1/500 dilution + Human Hsp90 alpha (amino acids 275-484) recombinant protein
Predicted band size: 85 kDa
Expected MWt of recombinant protein is 50.5 kDa. -
ELISA using ab128483.
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All lanes : Anti-Hsp90 alpha antibody [5G5] (ab128483) at 1/500 dilution
Lane 1 : NIH 3T3 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : HCT116 cell lysate
Lane 4 : HL-60 cell lysate
Lane 5 : SK-Br-3 cell lysate
Lane 6 : C6 cell lysate
Lane 7 : COS7 cell lysate
Predicted band size: 85 kDa -
ab128483, at a 1/200 dilution, staining Hsp90 alpha in HeLa cells by Flow Cytometry (green). Negative control is shown in red.
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ab128483, at a 1/200 dilution, staining Hsp90 alpha in paraffin embedded lung cancer tissue by Immunohistochemistry, with DAB staining.
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ab128483, at a 1/200 dilution, staining Hsp90 alpha in paraffin embedded lung cancer tissue by Immunohistochemistry, with DAB staining.
Protocols
Datasheets and documents
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Datasheet download
References (1)
ab128483 has been referenced in 1 publication.
- Wang Q & Liu X VDAC upregulation and aTAT1-mediated a-tubulin acetylation contribute to tanespimycin-induced apoptosis in Calu-1 cells. Oncol Rep 44:2725-2734 (2020). PubMed: 33125132