Overview

  • Product name
    Anti-HuC/HuD protein antibody
  • Description
    Rabbit polyclonal to HuC/HuD protein
  • Host species
    Rabbit
  • Tested applications
    Suitable for: IHC - Wholemountmore details
  • Species reactivity
    Reacts with: Zebrafish
  • Immunogen

    Recombinant fragment within Zebrafish HuC/HuD protein aa 48-345. The exact sequence is proprietary.
    Database link: P79736

  • Positive control
    • IHC-Wm: 2 days-post-fertilization zebrafish embryo, 24 hours-post-fertilization zebrafish embryo.

Properties

Applications

Our Abpromise guarantee covers the use of ab210554 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC - Wholemount 1/100 - 1/500.

Target

  • Function
    Binds to AU-rich sequences (AREs) of target mRNAs, including VEGF mRNA. May also bind poly-A tracts via RRM 3 (By similarity). May be involved in neuronal differentiation and maintenance.
  • Tissue specificity
    Brain specific.
  • Sequence similarities
    Belongs to the RRM elav family.
    Contains 3 RRM (RNA recognition motif) domains.
  • Domain
    RRM 1 and RRM 2 bind cooperatively to AU-rich sequences in target mRNAs. RRM 3 binds to poly-A mRNA sequences.
  • Information by UniProt
  • Database links
  • Alternative names
    • ELAV (embryonic lethal, abnormal vision, Drosophila) like 3 (Hu antigen C) antibody
    • ELAV (embryonic lethal, abnormal vision, Drosophila) like 4 (Hu antigen D) antibody
    • ELAV like 3 antibody
    • ELAV like 4 antibody
    • ELAV like neuron specific RNA binding protein 3 antibody
    • ELAV like neuron specific RNA binding protein 4 antibody
    • ELAV like protein 3 antibody
    • ELAV like protein 4 antibody
    • ELAV-like protein 3 antibody
    • ELAV3_HUMAN antibody
    • Elavl3 antibody
    • ELAVL4 antibody
    • Hu antigen C antibody
    • Hu antigen D antibody
    • Hu-antigen C antibody
    • HuC antibody
    • HUCL antibody
    • HuD antibody
    • Paraneoplastic cerebellar degeneration-associated antigen antibody
    • Paraneoplastic encephalomyelitis antigen HuD antibody
    • Paraneoplastic limbic encephalitis antigen 21 antibody
    • PLE21 antibody
    • PNEM antibody
    see all

Images

  • Wholemount Immunohistochemical analysis of 2 days-post-fertilization zebrafish embryo labeling HuC/HuD with ab210554 at 1/100 dilution (green); Tubulin (Acetyl Lys40) at 1/25 dilution (red).

  • Wholemount Immunohistochemical analysis of 2 days-post-fertilization zebrafish embryo labeling HuC/HuD with ab210554 at 1/100 dilution (green); Tubulin (Acetyl Lys40) at 1/25 dilution (red).

  • Wholemount Immunohistochemical analysis of 24 hours-post-fertilization zebrafish embryo labeling HuC/HuD with ab210554 at 1/100 dilution (green); Tubulin (Acetyl Lys40) at 1/25 dilution (red).

  • Wholemount immunohistochemical analysis of Paraformaldehyde-fixed zebrafish embryos labeling HuC/HuD with ab210554 at 1/200 dilution.

     

References

ab210554 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Testis, postnatal day 6)
Permeabilization
Yes - 0.1% Triton X-100 in PBS
Specification
Testis, postnatal day 6
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
Fixative
Paraformaldehyde

Mr. Bryan Niedenberger

Verified customer

Submitted Sep 14 2017

Application
Immunocytochemistry/ Immunofluorescence
Sample
Rat Cell (primary culture of enteric nervous system from rat)
Permeabilization
Yes - Triton X 100 0.5%
Specification
primary culture of enteric nervous system from rat
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 4% · Temperature: 25°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Sep 05 2016

Application
IHC - Wholemount
Sample
Human Tissue (Intestinal tissue)
Specification
Intestinal tissue

Abcam user community

Verified customer

Submitted Aug 22 2016

Application
IHC - Wholemount
Sample
Rat Tissue (Intestinal tissue)
Specification
Intestinal tissue

Mr. Julien Chevalier

Verified customer

Submitted Aug 19 2016

Application
IHC - Wholemount
Sample
Mouse Tissue (Intestinal tissue (myenteric plexus))
Specification
Intestinal tissue (myenteric plexus)

Mr. Julien Chevalier

Verified customer

Submitted Aug 19 2016

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