Overview

  • Product name

    Human Activin A ELISA Kit
    See all Activin A kits
  • Detection method

    Colorimetric
  • Sample type

    Cell culture supernatant, Serum, Plasma
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    < 15 pg/ml
  • Range

    20.48 pg/ml - 5000 pg/ml
  • Recovery

    83 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 94.73 82% - 111%
    Serum 77.89 68% - 83%
    Plasma 76.92 67% - 110%

  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Abcam’s Activin A Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Activin A in serum, plasma and cell culture supernatants.

    This assay employs an antibody specific for Human Activin A coated on a 96-well plate. Standards and samples are pipetted into the wells and Activin A present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Activin A antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Activin A bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimization may be required with urine samples.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

  • Storage instructions

    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    200X HRP-Streptavidin Concentrate 1 x 200µl
    20X Wash Buffer Concentrate 1 x 25ml
    5X Assay Diluent B 1 x 15ml
    Activin A Microplate (12 x 8 wells) 1 unit
    Assay Diluent A 1 x 30ml
    Biotinylated anti-Human Activin A (lyophilized) 2 vials
    Recombinant Human Activin A Standard (lyophilized)  2 vials
    Stop Solution 1 x 8ml
    TMB One-Step Substrate Reagent 1 x 12ml
  • Research areas

  • Relevance

    Activin A is a homodimer of 14kDa beta-A. Activin A, a cytokine member of the transforming growth factor-beta superfamily, is expressed locally by the mesenchymal component of the hemopoietic microenvironment. Its expression is regulated on the mRNA level by different cytokines, and the biological activity of the protein is tightly controlled by several inhibitory molecules.1 Inhibins and activins are members of the transforming growth factor beta superfamily and are known to modulate the growth and differentiation of several cell types.2 Inhibins and activins inhibit and activate, respectively, the secretion of follitropin by the pituitary gland. Inhibins/activins are involved in regulating a number of diverse functions such as hypothalamic and pituitary hormone secretion, gonadal hormone secretion, germ cell development and maturation, erythroid differentiation, insulin secretion, nerve cell survival, embryonic axial development or bone growth, depending on their subunit composition. Inhibins appear to oppose the functions of activins. The standard product used in this kit is recombinant Activin A, which is composed of two single chains of 116 amino acids with the molecular mass of 26KDa.
  • Cellular localization

    Secreted
  • Alternative names

    • Activin beta A chain
    • Beta 1
    • erythroid differentiation factor
    • erythroid differentiation protein
    • follicle stimulating hormone releasing protein
    • FSH releasing protein
    • Inhibin
    • inhibin beta A chain
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab113316 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Hu Activin A measured in biological fluids showing quantity (pg) per mLof tested sample.

  • Representative standard curve using ab113316

  • Representative standard curve using ab113316

Protocols

References

This product has been referenced in:

  • Kudryashova TV  et al. Inhibitory Antibodies against Activin A and TGF-ß Reduce Self-Supported, but Not Soluble Factors-Induced Growth of Human Pulmonary Arterial Vascular Smooth Muscle Cells in Pulmonary Arterial Hypertension. Int J Mol Sci 19:N/A (2018). Read more (PubMed: 30274147) »
See 1 Publication for this product

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

straight forward ELISA for Activin A

Excellent Excellent 5/5 (Ease of Use)
Abreviews
Used this kit to measure activin a in several human plasma samples. All samples were diluted with assay diluent A in a 1:2 dilution as recommended in the protocol.
The ELISA is very easy, requires no coating and can be done for a few samples at a time, as it contains 12 strips.
Very clear standard curve and all samples came up with a good signal too.
Will use again.

Abcam user community

Verified customer

Submitted May 22 2015

Activin A Human Elisa Kit

Excellent Below average 2/5 (Ease of Use)
Abreviews
As per usual, the Elisa Kits from Abcam are very easy to use and the experimental protocol doesn't take too long to be completed.
Our lab is very satisfied with this product and we would strongly recommend it.

Abcam user community

Verified customer

Submitted Aug 21 2014

Answer

Thank you for your inquiry.

I can confirm that both kits come with strips (12 x 8) and not a plate.

I can fully recommend both kit for the measurement of humanActivin A by ELISA.

The only majordifference is that ab113316 is not tested and guaranteed for tissue extract samples. If you are using tissue samples, I suggest to choose ab119568.

I hope this information is helpful. Please do not hesitate to contact me again with any further questions.

Read More

Answer

Thank you for contacting us.

Unfortunately, the clone numbers of the antibodies are proprietary so this information is not available.

The kit will detect recombinant human Activin A and also the Activin A precursor. Both antibodies used are specific for the Activin A βA subunit. The kit is detecting free Activin A however it is not known whether it will also recognize Activin A bound to follistatin.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More

Answer

Thank you for your enquiry and interest.
I regret to inform you that the clone numbers of the antibodies are proprietary so this information is not available.
However, below is some specificity information that the customer may find useful. The kit (ab113316: Activin A Human ELISA) will detect recombinant human Activin A and also the Activin A precursor. Both antibodies used are specific for the Activin A βA subunit. The kit is detecting free Activin A, however it is not known whether it will also recognize Activin A bound to follistatin.
I hope this information will be useful for you.

Read More

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