Human Aggrecan ELISA Kit (ab213754)
- Datasheet
- References
- Protocols
Overview
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Product name
Human Aggrecan ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% 1 16 15ng/ml 0.825 5.5% 2 16 42ng/ml 1.806 4.3% 3 16 74ng/ml 4.588 6.2% Inter-assay Sample n Mean SD CV% 1 24 16ng/ml 0.944 5.9% 2 24 41ng/ml 2.255 5.5% 3 24 72ng/ml 4.824 6.7% -
Sample type
Cell culture supernatant, Urine, Serum -
Assay type
Sandwich (quantitative) -
Sensitivity
< 50 pg/ml -
Range
1.56 ng/ml - 100 ng/ml -
Assay time
3h 30m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
The Human Aggrecan Enzyme-Linked Immunosorbent Assay (ELISA) kit (ab213754) is designed for the quantitative measurement of Human Aggrecan in cell culture supernatants, serum and urine.
The ELISA kit is based on standard sandwich enzyme-linked immunosorbent assay technology. A monoclonal antibody from mouse specific for Aggrecan has been pre-coated onto 96-well plates. Standards (NSO, V20-G675) and test samples are added to the wells, a biotinylated detection monoclonal antibody from mouse specific for Aggrecan is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. HRP substrate TMB is used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Human Aggrecan amount of sample captured in plate.
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Notes
Aggrecan, also known as cartilage-specific proteoglycan core protein (CSPCP) or chondroitin sulfate proteoglycan 1, is a protein that in humans is encoded by the ACAN gene. It is a critical component for cartilage structure and the function of joints. Aggrecan provides intervertebral disc and cartilage with the ability to resist compressive loads. The localized high concentrations of aggrecan provide the osmotic properties necessary for normal tissue function with the GAGs producing the swelling pressure that counters compressive loads on the tissue. This functional ability is dependent on a high GAG/aggrecan concentration being present in the tissue extracellular matrix. Also, Aggrecan plays an important role in the organization of the extracellular spaces between neurons in the brain.
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Tested applications
Suitable for: Sandwich ELISAmore details -
Platform
Pre-coated microplate (12 x 8 well strips)
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 1 x 96 tests ABC Diluent Buffer Blue Cap 1 x 12ml Adhesive Plate Seal 4 units Antibody Diluent Buffer Green Cap 1 x 12ml Anti-Human Aggrecan coated Microplate (12 x 8 wells) 1 unit Avidin-Biotin-Peroxidase Complex (ABC) 1 x 130µl Biotinylated anti- Human Aggrecan antibody 1 x 130µl Lyophilized recombinant Human Aggrecan standard 2 vials Sample Diluent Buffer Green Cap 1 x 30ml TMB Color Developing Agent Black Cap 1 x 10ml TMB Stop Solution Yellow Cap 1 x 10ml -
Research areas
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Relevance
Aggrecan is a member of a family of large, aggregating proteoglycans (also including versican, brevican and neurocan) which is found in articular cartilage. Aggrecan is composed of three major domains: G1, G2, and G3. Between the G1 and G2 domains there is an interglobulin region (IGD). The IGD region is the major site of cleavage by specific proteases like metalloproteinases (MMPs) and aggrecanase. Aggrecan cleavage has been associated with a number of degenerative diseases including rheumatoid arthritis and osteoarthritis. There is evidence that this family of proteoglycans modulates cell adhesion, migration, and axonal outgrowth in the CNS. -
Cellular localization
Secreted; extracellular matrix. -
Alternative names
- ACAN
- AGC 1
- AGC1
see all -
Database links
- Entrez Gene: 176 Human
- Omim: 155760 Human
- SwissProt: P16112 Human
Applications
Our Abpromise guarantee covers the use of ab213754 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Sandwich ELISA | Use at an assay dependent concentration. |
Images
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Human Aggrecan ELISA Kit (ab213754) Standard Curve.Human Aggrecan ELISA Kit (ab213754) Standard Curve.
Protocols
References
ab213754 has not yet been referenced specifically in any publications.
