Overview

  • Product name
    Human Apolipoprotein AII ELISA Kit, Fluorescent
    See all Apolipoprotein A II/ApoA-II kits
  • Detection method
    Fluorescent
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Standard 5 2%
    Inter-assay
    Sample n Mean SD CV%
    Cell extract 3 10.6%
  • Sample type
    Cell culture supernatant, Urine, Serum, Heparin Plasma, EDTA Plasma, Citrate Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    7.2 pg/ml
  • Range
    7.8 pg/ml - 4000 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Urine 90.2 89.3% - 90.8%
    Cell culture media 125.2 122.2% - 127.8%

  • Assay time
    1h 30m
  • Assay duration
    One step assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Apolipoprotein AII (ApoA2) in vitro CatchPoint SimpleStep ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Apolipoprotein AII (ApoA2) protein in human serum, plasma, urine, and cell culture supernatant samples.


    This CatchPoint SimpleStep ELISA kit has been optimized for Molecular Devices Microplate Readers. Click here for a list of recommended Microplate Readers.
    If using a Molecular Devices’ plate reader supported by SoftMax® Pro software, a preconfigured protocol for these CatchPoint SimpleStep ELISA Kits is available with all the protocol and analysis settings at www.softmaxpro.org.


    The CatchPoint SimpleStep ELISA employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material.  CatchPoint HRP Development Solution containing the Stoplight Red Substrate is added. During incubation, the substrate is catalyzed by HRP generating a fluorescent product. Signal is generated proportionally to the amount of bound analyte and the intensity is measured in a fluorescence plater reader at 530/570/590 nm Excitation/Cutoff/Emission.

  • Notes

    Apolipoprotein AII may stabilize HDL (high density lipoprotein) structure by its association with lipids, and affect the HDL metabolism. ApoA2 forms a disulfide-linked homodimer. Apolipoprotein AII also forms a disulfide-linked heterodimer with APOD and interacts with HCV core protein, APOA1BP and NDRG1.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Pre-coated microplate (12 x 8 well strips)

Properties

Applications

Our Abpromise guarantee covers the use of ab229423 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • ELISA Protocol Summary
  • Background-subtracted data values (mean +/- SD) are graphed.

  • Background-subtracted data values (mean +/- SD) are graphed.

  • Interpolated data values corrected for sample dilution are graphed in μg of ApoA2 per mL of serum (mean +/- SD, n=3). The mean of ApoA2 concentration of these ten individual serum samples was determined to be 209 µg/mL with a range of 143 – 225 µg/mL.

Protocols

References

ab229423 has not yet been referenced specifically in any publications.

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