Overview

  • Product name

    Human Apolipoprotein H ELISA Kit (APOH)
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall 4.2%
    Inter-assay
    Sample n Mean SD CV%
    Overall 10%
  • Sample type

    Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma, Cell culture extracts, Cell Lysate
  • Assay type

    Sandwich (quantitative)
  • Sensitivity

    = 0.45 ng/ml
  • Range

    0.625 ng/ml - 40 ng/ml
  • Recovery

    98 %

  • Assay time

    4h 00m
  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Abcam’s Apolipoprotein H (APOH) Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of Apolipoprotein H concentrations in Human plasma, serum, saliva, urine, milk and cell culture samples.


    An Apolipoprotein H specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently an Apolipoprotein H specific biotinylated detection antibody is added and then followed by washing with wash buffer. Streptavidin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize Streptavidin-Peroxidase enzymatic reaction. TMB is catalyzed by Streptavidin-Peroxidase to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the amount of Apolipoprotein H captured in plate.


    The entire kit may be stored at -20°C for long term storage before reconstitution - Avoid repeated freeze-thaw cycles.

  • Tested applications

    Suitable for: Sandwich ELISAmore details
  • Platform

    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab108814 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent dilution.

Images

  • Apolipoprotein H measured in biological fluids with background signal subtracted (duplicates +/- SD).The samples were tested in a range of dilutions (human serum and plasma at 1:10000-1:200000; milk at 1:100-1:1000 and the rest 1:1-1:50).

  • Representative Standard Curve using ab108814

Protocols

References

ab108814 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer



Our guarantee period is 12 month from the date of purchase and therefore I suggest to also take this date as expiry date for un-opened kits that are stored as described in the protocol booklet.

When reagents are opened they also can be stored for a short vial (please refer to the protocol booklets) but they will expire very shortly after openeing.

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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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