Key features and details
- Sensitivity: 2 pg/ml
- Range: 2.46 pg/ml - 600 pg/ml
- Sample type: Cell culture supernatant, Plasma
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Product nameHuman CD262 ELISA Kit (TRAIL R2)
Sample typeCell culture supernatant, Plasma
Assay typeSandwich (quantitative)
Sensitivity< 2 pg/ml
Range2.46 pg/ml - 600 pg/ml
Sample specific recovery Sample type Average % Range Cell culture supernatant 97.22 88% - 107% Plasma 78.79 68% - 97%
Assay durationMultiple steps standard assay
Species reactivityReacts with: Human
Abcam’s CD262 (TRAIL R2) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human CD262 in plasma and cell culture supernatants. (Human CD262 concentration is pretty low in normal plasma, it may not be detected in this assay).
This assay employs an antibody specific for Human CD262 coated on a 96-well plate. Standards and samples are pipetted into the wells and CD262 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human CD262 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of CD262 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
We have not been able to detect endogenous Human CD262 in normal serum with ab113340, only in serum spiked with Human CD262.
Optimization may be required with urine samples.
Storage instructionsStore at -20°C. Please refer to protocols.
Components 1 x 96 tests 200X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer Concentrate 1 x 25ml 5X Assay Diluent B 1 x 15ml Assay Diluent A 1 x 30ml Biotinylated anti-Human CD262 2 vials CD262 Microplate (12 x 8 wells) 1 unit Recombinant Human CD262 Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml
- SwissProt: O14763 Human
ab113340 has not yet been referenced specifically in any publications.