Overview

  • Product name
    Human Chorionic Gonadotropin beta ELISA Kit
    See all Chorionic Gonadotropin beta kits
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall 48 < 7.6%
    Inter-assay
    Sample n Mean SD CV%
    Overall 3 < 8.8%
  • Sample type
    Serum, Heparin Plasma, Citrate Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    > 0.09 mU/ml
  • Range
    1 mU/ml - 400 mU/ml
  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Chorionic Gonadotropin beta in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Chorionic Gonadotropin beta in Human serum and plasma.

    A 96-well plate has been precoated with anti-Chorionic Gonadotropin beta IgG antibodies. Samples, standards and the Chorionic Gonadotropin beta-HRP conjugate are added to the wells, where Chorionic Gonadotropin beta in the sample and standards binds to the precoated antibody and added Chorionic Gonadotropin beta-HRP conjugate binds to this antibody-Chorionic Gonadotropin beta complex. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is directly proportional to the amount of Chorionic Gonadotropin beta in the sample and the intensity is measured at 450 nm.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

  • Storage instructions
    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Anti-Chorionic Gonadotropin beta HRP Conjugate 1 x 1ml
    50X Washing Solution 1 x 20ml
    Anti-Chorionic Gonadotropin beta Coated Microplate (12 x 8 wells) 1 unit
    Chorionic Gonadotrophin beta Control 1 x 1ml
    Chorionic Gonadotropin beta Standard 0 - 0 mU/mL 1 x 1ml
    Chorionic Gonadotropin beta Standard 1 - 1 mU/mL 1 x 1ml
    Chorionic Gonadotropin beta Standard 2 - 5 mU/mL 1 x 1ml
    Chorionic Gonadotropin beta Standard 3 - 20 mU/mL 1 x 1ml
    Chorionic Gonadotropin beta Standard 4 - 100 mU/mL 1 x 1ml
    Chorionic Gonadotropin beta Standard 5 - 400 mU/mL 1 x 1ml
    Cover Foil 1 unit
    Incubation Buffer 1 x 50ml
    Stop Solution 1 x 15ml
    Strip holder 1 unit
    TMB Substrate Solution 1 x 15ml
  • Research areas
  • Database links

Applications

Our Abpromise guarantee covers the use of ab178633 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Protocols

References

ab178633 has not yet been referenced specifically in any publications.

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