• Product name
    Human ErbB2 ELISA Kit
  • Detection method
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 8 pg/ml
  • Range
    8.19 pg/ml - 2000 pg/ml
  • Recovery

    > 81 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 82.77 70% - 96%
    Serum 91.93 76% - 106%
    Plasma 81.32 69% - 97%

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s ErbB2 (Epidermal Factor Growth Factor Receptor 2) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of human ErbB2 in serum, plasma, and cell culture supernatants.

    This assay employs an antibody specific for human ErbB2 coated on a 96-well plate. Standards and samples are pipetted into the wells and ErbB2 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-human ErbB2 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of ErbB2 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Notes

    Optimisation may be required with urine samples.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform



Our Abpromise guarantee covers the use of ab100510 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.


  • Representative Standard Curve using ab100510.

  • Representative Standard Curve using ab100510.



ab100510 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

HER2 ELISA KIT used for testing HER2 in the urine

Excellent Excellent 5/5 (Ease of Use)
We wanted to measure HER2 in the urine, using the Abcam HER2 ELISA kit. We did not dilute the urine samples and we were able to detect the levels of the protein using the kit. The concentrations of HER2 in the urine of healthy individuals are really low (below 100 pg/ml), so the sensitivity of the kit is a major issue. The Abcam HER2 ELISA kit had a better sensitivity than other kits available in the market.

Abcam user community

Verified customer

Submitted Dec 09 2013


Thank you very much for your interest in ab100510.

To our knowledge, ab100510 has not been tested in monkey samples, howvever there is a 97% level of homology between the human and monkey sequences, therefore there is a a good chance the kit will work for you. Therefore, I can offer a discount off a future purchase if you buy ab100510 now, test it in monkey samples and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of: $470 VALUE OFF OF NEXT ORDER.

If you are interested in this offer, please follow these steps:

1. Reply to this e-mail to let me know that you would like to proceed and test ab100510 in monkey samples. I will then send a discount code. This code must be issued before purchasing ab100510 so please wait for my reply before ordering.

2. Purchase ab100510 either by phone, fax, or online (www.abcam.com).

3. Test it in monkey samples.

4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out how to submit an Abreview, please visit: https://www.abcam.com/abreviews.

5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any$470 VALUE OFF OF NEXT ORDER ordered and the discount code is valid for 4 months after issue.
Please remember that submission of the Abreview is sufficient for the discount code to become active.

We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if ab100510 turns out to be unsuitable for monkey samples, you will still receive the discount on your next purchase after your Abreview has been submitted.

Please let me know if you have any questions about this offer and I would be happy to help you further.

The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

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The capture antibody is monoclonal and from mouse, while the detection antibody is polyclonal and from goat. Both antibodies detect the extracellular domain of ErbB2.

I hope this is helpful. Please let me know if you need further assistance.

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Thank you for contacting us.  Yes, the customer could certainly use the included antibody and reagents to detect ErbB 2 in a test sample that has been directly coated to an empty ELISA plate.  If the customer has the detection reagents, he or she could also just use a primary antibody alone without purchasing the kit.  I hope this helps, please let me know if you need any additional information. 

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Thank you for contacting us. The standard provided with this kit corresponds to amino acids Thr23 - Thr652, which is the extracellular domain of ErbB2. Unfortunately, the antibodies for this kit are not currently available to purchase separately and their immunogens are considered proprietary. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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