Human FGF basic ELISA Kit (FGF2) (ab99979)
Key features and details
- Sensitivity: 50 pg/ml
- Range: 102.4 pg/ml - 10000 pg/ml
- Sample type: Cell culture supernatant, Cell Lysate, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human FGF basic ELISA Kit (FGF2)
See all FGF basic kits -
Detection method
Colorimetric -
Sample type
Cell culture supernatant, Serum, Plasma, Cell Lysate -
Assay type
Sandwich (quantitative) -
Sensitivity
< 50 pg/ml -
Range
102.4 pg/ml - 10000 pg/ml -
Recovery
94 %
Sample specific recovery Sample type Average % Range Cell culture supernatant 93.48 83% - 103% Serum 95.46 84% - 104% Plasma 92.64 82% - 103% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human
Predicted to work with: Pig -
Product overview
Abcam’s FGF basic (FGF2) Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human FGF basic in serum, plasma, and cell culture supernatants.
This assay employs an antibody specific for Human FGF basic coated on a 96-well plate. Standards and samples are pipetted into the wells and FGF basic present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human FGF basic antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of FGF basic bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Notes
Optimization may be required with urine samples.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 120X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer 1 x 25ml 5X Assay Diluent B 1 x 15ml Assay Diluent A 1 x 30ml Biotinylated anti-Human FGF basic 2 vials FGF basic Microplate (12 x 8 wells) 1 unit Recombinant Human FGF basic Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Relevance
FGF basic (FGF2) is a member of the fibroblast growth factor (FGF) family. FGF family members bind heparin and possess broad mitogenic and angiogenic activities. FGF2 is involved in diverse biological processes, such as limb and nervous system development, wound healing, and tumor growth. FGF2 mRNA contains multiple polyadenylation sites, and is alternatively translated from AUG and non-AUG (CUG) initiation codons resulting in five different isoforms with distinct properties. The CUG-initiated isoforms are localized in the nucleus and are responsible for the intracrine effect, whereas, the AUG-initiated form is mostly cytosolic and is responsible for the paracrine and autocrine effects of this FGF. -
Alternative names
- Basic fibroblast growth factor
- BFGF
- FGF 2
see all -
Database links
- Entrez Gene: 2247 Human
- Omim: 134920 Human
- SwissProt: P09038 Human
Datasheets and documents
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SDS download
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Datasheet download
References (13)
ab99979 has been referenced in 13 publications.
- Wang J et al. Inhibition of PLK4 might enhance the anti-tumour effect of bortezomib on glioblastoma via PTEN/PI3K/AKT/mTOR signalling pathway. J Cell Mol Med 24:3931-3947 (2020). PubMed: 32126150
- Yuan YF et al. Cyclopamine functions as a suppressor of benign prostatic hyperplasia by inhibiting epithelial and stromal cell proliferation via suppression of the Hedgehog signaling pathway. Int J Mol Med 46:311-319 (2020). PubMed: 32319534
- Kaneko Y et al. Rhynchophylline promotes stem cell autonomous metabolic homeostasis. Cytotherapy 22:106-113 (2020). PubMed: 31983606
- Matsumura T et al. TGF-ß-induced activation of conjunctival fibroblasts is modulated by FGF-2 and substratum stiffness. PLoS One 15:e0242626 (2020). PubMed: 33206726
- Wan X et al. Hypoxia-preconditioned adipose-derived stem cells combined with scaffold promote urethral reconstruction by upregulation of angiogenesis and glycolysis. Stem Cell Res Ther 11:535 (2020). PubMed: 33308306