• Product name
    Human G-CSF ELISA Kit
    See all G-CSF kits
  • Detection method
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 1 pg/ml
  • Range
    0.69 pg/ml - 500 pg/ml
  • Recovery

    > 75 %

    Sample specific recovery
    Sample type Average % Range
    Cell culture supernatant 76.56 68% - 90%
    Serum 83.45 72% - 94%
    Plasma 74.76 67% - 87%

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s G-CSF Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human G-CSF in serum, plasma, and cell culture supernatants.

    This assay employs an antibody specific for Human G-CSF coated on a 96-well plate. Standards and samples are pipetted into the wells and G-CSF present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human G-CSF antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of G-CSF bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.

    Get results in 90 minutes with Human G-CSF ELISA Kit (CSF3) (ab188390) from our SimpleStep ELISA® range.


  • Notes

    Optimization may be required with urine samples

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform


  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    20X Wash Buffer Concentrate 1 x 25ml
    260X HRP-Streptavidin Concentrate 1 x 200µl
    5X Assay Diluent B 1 x 15ml
    Assay Diluent A 1 x 30ml
    Biotinylated anti-Human G-CSF (lyophilized) 2 vials
    G-CSF Microplate (12 x 8 wells) 1 unit
    Recombinant Human G-CSF Standard (lyophilized) 2 vials
    Stop Solution 1 x 8ml
    TMB One-Step Substrate Reagent 1 x 12ml
  • Research areas
  • Relevance
    Granulocyte Colony Stimulating Factor (G-CSF) a cytokine that controls the production, differentiation, and function of granulocytes. It is a potent stimulator of bone marrow cells especially those of neutrophil lineage. In addition, G-CSF can enhance the survival and activate the immunological functions of mature neutrophils. Recombinant human G-CSF has been developed by pharmaceutical companies, and since the late 1980's, multiple clinical trials have explored its efficacy in a variety of conditions.
  • Alternative names
    • C17orf33
    • Colony stimulating factor 3 (granulocyte)
    • CSF 3
    • CSF beta
    • CSF3
    • CSF3OS
    • Csfg
    • Filgrastim
    • GCSA
    • GCSF
    • Granulocyte colony stimulating fact
    • Lenograstim
    • Macrophage granulocyte inducer 2
    • MGC45931
    • MGI 2
    • MGI IG
    • OTTMUSP00000006785
    • Pluripoietin
    • RP23-395E10.4
    see all
  • Database links

Associated products


Our Abpromise guarantee covers the use of ab100524 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.


  • Standard curve in dilutent B with background signal subtracted (duplicates; +/- SD).

  • Human G-CSF measured in undiluted culture supernatants (duplicates +/- SD).

  • Representative standard curve using ab100524

  • G-CSF measured in undiluted human plasma, signal in serum was below level of detection (duplicates +/- SD).

  • Representative standard curve using ab100524



ab100524 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

We have not tested the GCSF ELISA kit with Cynomolgus monkey serum. However, when looking at the human GCSF sequence our kit recognizes and testing the cross-reactivity to the Cynomolgus sequence, there are a number of predicted matches, including gran...

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The standard recombinant protein of ab100524 was expressed in E.coli.

The standard we use for this kit (ab100524) is recombinant human G-CSF: 18.7 kDa protein; 174 amino acid residues. We have not tested this kit with a met-GCSF, but the following amino acid sequence of the recombinant standard used should help the custo...

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Thank you for contacting us.

Serum can be stored at -80C for a month, this will be having minimal affect on the assay.

The following publication will help understanding the affect of temperature on different serum protein.


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Thank you for your reply.

The strips come with a rack where the single strips can be inserted.

This rack will fit all standard plate readers.

I am sorry, I cannot check if this is also true for your plate reader. Please as...

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Thank you for your inquiry.

I can confirm that with ab100524 it is not necessary to use the whole plate at one time.

The plate comes in convenient 12 strips x 8 wells.

Please follow the instruction of the protocol when sto...

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Thank you for contacting Abcam.

We still have not tested whether the kit can be used to quantitate PEGfilgrastim, therefore the original response, "to spike in pegylated G-CSF and see" is still the only option for answering this que...

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Thank you for your inquiry.  That is a good question.  I reviewed our testing records on ab100524 but unfortunately I was not able to find any data about pegylated G-CSF.  Therefore we don’t know if this kit will also detect the pegylated form....

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