Human HCC-1 ELISA Kit (ab155433)
Key features and details
- Sensitivity: 7 pg/ml
- Range: 0.006 ng/ml - 1.5 ng/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human HCC-1 ELISA Kit -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall < 10% Inter-assay Sample n Mean SD CV% Overall < 12% -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
< 7 pg/ml -
Range
0.006 ng/ml - 1.5 ng/ml -
Recovery
Sample specific recovery Sample type Average % Range Serum 108.4 102% - 114% Plasma 102.1 90% - 108% Cell culture media 110 105% - 125% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam's HCC-1 Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay designed for the quantitative measurement of Human HCC-1 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Human HCC-1 coated on a 96-well plate. Standards and samples are pipetted into the wells and HCC-1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human HCC-1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Notes
Optimization may be required with urine samples.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer 1 x 25ml 400X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml Assay Diluent A 1 x 30ml Biotinylated anti-Human HCC-1 2 vials HCC-1 Microplate (12 x 8 wells) 1 unit Recombinant Human HCC-1 Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Relevance
Has weak activities on human monocytes and acts via receptors that also recognize MIP-1 alpha. It induced intracellular Ca2+ changes and enzyme release, but no chemotaxis, at concentrations of 100-1,000 nM, and was inactive on T-lymphocytes, neutrophils, and eosinophil leukocytes. Enhances the proliferation of CD34 myeloid progenitor cells. The processed form HCC-1(9-74) is a chemotactic factor that attracts monocytes eosinophils, and T-cells and is a ligand for CCR1, CCR3 and CCR5. -
Cellular localization
Secreted -
Alternative names
- C-C motif chemokine 14
- CC-1
- CC-3
see all -
Database links
- Entrez Gene: 6358 Human
- Omim: 601392 Human
- SwissProt: Q16627 Human
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab155433 has not yet been referenced specifically in any publications.