Other (Dot Blot) Abreview for Histone H3 peptide - di methyl K79


Review text: I used this product to do a dot blot specificity test for cross reactivity of modified histone antibodies and everything worked great. I made two dilutions of the peptide and spotted it on a nitrocellulose membrane using a Bio-Dot apparatus. Once the peptides were bound to the membrane I incubated with the appropriate primary and secondary antibodies at an assay dependent concentration and developed using ECL.
Sample: Human Purified protein

Primary antibody (in addition to 'Histone H3 peptide - di methyl K79')
Name: Non-Abcam antibody was used: anti-H3K79me2
Host species: Rabbit
Clonality: Polyclonal
Dilution: 1/3000

Secondary antibody
Name: Non-Abcam antibody was used: anti-rabbit
Host species: Mouse
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution: 1/10000

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Submitted Nov 03 2010

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