Overview

  • Product name
    Human IL-12 p70 ELISA Kit, Fluorescent
  • Detection method
    Fluorescent
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Spiked serum 5 7%
    Inter-assay
    Sample n Mean SD CV%
    Spiked serum 3 5.2%
  • Sample type
    Cell culture supernatant, Serum, Heparin Plasma, EDTA Plasma, Citrate Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    1 pg/ml
  • Range
    3.9 pg/ml - 8000 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 96 93% - 101%
    Cell culture media 101 94% - 110%
    Heparin Plasma 85 80% - 89%
    EDTA Plasma 99 97% - 101%
    Citrate Plasma 93 92% - 94%

  • Assay time
    1h 30m
  • Assay duration
    One step assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    IL-12 p70 in vitro CatchPoint SimpleStep ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-12 p70 protein in human serum, plasma and cell culture supernatant.


    This CatchPoint SimpleStep ELISA kit has been optimized for Molecular Devices Microplate Readers. Click here for a list of recommended Microplate Readers.
    If using a Molecular Devices’ plate reader supported by SoftMax® Pro software, a preconfigured protocol for these CatchPoint SimpleStep ELISA Kits is available with all the protocol and analysis settings at www.softmaxpro.org.


    The CatchPoint SimpleStep ELISA employs an affinity tag labeled capture antibody and a reporter conjugated detector antibody which immunocapture the sample analyte in solution. This entire complex (capture antibody/analyte/detector antibody) is in turn immobilized via immunoaffinity of an anti-tag antibody coating the well. To perform the assay, samples or standards are added to the wells, followed by the antibody mix. After incubation, the wells are washed to remove unbound material. CatchPoint HRP Development Solution containing the Stoplight Red Substrate is added. During incubation, the substrate is catalyzed by HRP generating a fluorescent product. Signal is generated proportionally to the amount of bound analyte and the intensity is measured in a fluorescence plater reader at 530/570/590 nm Excitation/Cutoff/Emission.

  • Notes

    IL-12 p70 is a 70kDa cytokine heterodimer made of 2 subunits IL-12A (p35) and IL-12B (p40). This kit recognizes specifically the IL-12 total heterodimer protein. IL-12 p70 is produced by active antigen-presenting cells and promotes the development of Th1 and induces IFNγ.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions
    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    100X Stoplight Red Substrate 1 x 120µl
    10X Wash Buffer PT 1 x 20ml
    500X Hydrogen Peroxide (H2O2, 3%) 1 x 50µl
    Antibody Diluent CPI 1 x 6ml
    10X Human IL-12 p70 Capture Antibody 1 x 600µl
    10X Human IL-12 p70 Detector Antibody 1 x 600µl
    Human IL-12 p70 Lyophilized Recombinant Protein 2 vials
    Plate Seals 1 unit
    Sample Diluent NS 1 x 50ml
    SimpleStep Pre-Coated Black 96-Well Microplate 1 unit
    Stoplight Red Substrate Buffer 1 x 12ml
  • Research areas
  • Relevance
    IL-12 p70 is a disulfide bonded heterodimer of the IL-12 40 kDa and 35 kDa subunits.
  • Cellular localization
    Secreted
  • Alternative names
    • IL12A
    • IL12B
    • CLMF p35
    • CLMF p40
    • Cytotoxic lymphocyte maturation factor 35 kDa subunit
    • Cytotoxic lymphocyte maturation factor 40 kDa subunit
    • Interleukin 12 alpha chain
    • Interleukin-12 beta chain
    • NK cell stimulatory factor chain 1
    • NK cell stimulatory factor chain 2
    • NKSF1
    • NKSF2
    see all
  • Database links

Applications

Our Abpromise guarantee covers the use of ab229515 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • ELISA Protocol Summary
  • The IL-12 p70 standard curve was prepared as described in Section 10. Background-subtracted data values (mean +/- SD) are graphed.

  • The concentrations of IL-12 p70 were measured in duplicates, interpolated from the IL-12 p70 standard curves and corrected for sample dilution. Undiluted samples are as follows: serum 50%, plasma (EDTA) 50%, plasma (heparin) 50%, plasma (citrate) 50% and cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

    Serum from ten individual healthy human female donors was measured in duplicate. No detectable levels of IL-12 p70 was measured.

Protocols

References

ab229515 has not yet been referenced specifically in any publications.

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