Human IL-17A Receptor ELISA Kit (ab100558)
Key features and details
- Sensitivity: 10 pg/ml
- Range: 12.35 pg/ml - 3000 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human IL-17A Receptor ELISA Kit -
Detection method
Colorimetric -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
< 10 pg/ml -
Range
12.35 pg/ml - 3000 pg/ml -
Recovery
81 %
Sample specific recovery Sample type Average % Range Cell culture supernatant 81.54 70% - 92% Serum 87.64 83% - 104% Plasma 73.27 67% - 87% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s IL-17A Receptor Human ELISA (Enzyme Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human IL-17A in serum, plasma, and cell culture supernatants.
This assay employs an antibody specific for Human IL-17A coated on a 96-well plate. Standards and samples are pipetted into the wells and IL-17A present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human IL-17A antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of IL-17A bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
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Notes
Optimization may be required with urine samples.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 200X HRP-Streptavidin Concentrate 1 x 200µl 20X Wash Buffer 1 x 25ml 5X Assay Diluent B 1 x 15ml Assay Diluent C 1 x 30ml Biotinylated anti-Human IL-17A 2 vials IL-17A Microplate (12 x 8 wells) 1 x 200µl Recombinant Human IL-17A Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Function
Receptor for IL17A and IL17F. Binds its IL17A ligand with low affinity, suggesting that additional components are involved in IL17A-induced signaling. -
Tissue specificity
Widely expressed. -
Involvement in disease
Defects in IL17RA are the cause of familial candidiasis type 5 (CANDF5) [MIM:613953]. CANDF5 is a rare disorder with altered immune responses and impaired clearance of fungal infections, selective against Candida. It is characterized by persistent and/or recurrent infections of the skin, nails and mucous membranes caused by organisms of the genus Candida, mainly Candida albicans. -
Sequence similarities
Contains 1 SEFIR domain. -
Post-translational
modificationsGlycosylated. -
Cellular localization
Membrane. - Information by UniProt
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Alternative names
- CANDF5
- CD217
- CD217 antigen
see all -
Database links
- Entrez Gene: 23765 Human
- Omim: 605461 Human
- SwissProt: Q96F46 Human
- Unigene: 48353 Human
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab100558 has not yet been referenced specifically in any publications.