Human IL-6 ELISA Kit (ab46027)
Key features and details
- Sensitivity: 2 pg/ml
- Range: 6.25 pg/ml - 200 pg/ml
- Sample type: Cell culture supernatant, Plasma, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human IL-6 ELISA Kit
See all IL-6 kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Pooled sampl 15 4.2% Inter-assay Sample n Mean SD CV% Sample 1 18 200pg/ml 16 8.1% Sample 2 18 77pg/ml 8 10.1% Sample 3 18 63pg/ml 6 8.6% Sample 4 18 103pg/ml 6 6% Sample 5 18 53pg/ml 3 5.5% -
Sample type
Cell culture supernatant, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
< 2 pg/ml -
Range
6.25 pg/ml - 200 pg/ml -
Recovery
96 %
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Assay time
1h 45m -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s IL-6 Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of IL-6 in Human serum, plasma, buffered solutions or supernatants.
A monoclonal antibody specific for IL-6 has been coated onto the wells of the microtiter strips provided. Samples, including standards of known IL-6 concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for IL-6 are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of IL-6 present in the samples.
This kit will recognize both endogenous and recombinant Human IL-6.
Get higher sensitivity in only 90 minutes with Human IL-6 ELISA Kit (ab178013) from our SimpleStep ELISA® range.
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Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components Identifier 2 x 96 tests 1 x 96 tests 1 x 96 tests 2 x 96 tests 10X Standard Diluent Buffer Black 1 x 25ml 1 x 15ml 1 x 15ml 1 x 25ml 200X Wash Buffer White 2 x 10ml 1 x 10ml 1 x 10ml 2 x 10ml Adhesive plate seal 1 x 4 units 1 x 2 units 1 x 2 units 1 x 4 units Biotinylated Antibody Diluent Red 1 x 13ml 1 x 7.5ml 1 x 7.5ml 1 x 13ml Biotinylated anti-IL-6 Red 2 x 0.4ml 1 x 0.4ml 1 x 0.4ml 2 x 0.4ml Chromogen TMB Substrate Solution 1 x 24ml 1 x 11ml 1 x 11ml 1 x 24ml HRP Diluent Red 1 x 23ml 1 x 12ml 1 x 12ml 1 x 23ml IL-6 Control Yellow 4 vials 2 vials 2 vials 4 vials IL-6 Microplate (12 x 8 well strips) 2 units 1 unit 1 unit 2 units IL-6 Standard (Lyophilized) Orange 4 vials 2 vials 2 vials 4 vials Standard Diluent (Serum) 2 x 7ml 1 x 7ml 1 x 7ml 2 x 7ml Stop Reagent Black 2 x 11ml 1 x 11ml 1 x 11ml 2 x 11ml Streptavidin-HRP 4 x 5µl 2 x 5µl 2 x 5µl 4 x 5µl -
Research areas
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Function
Cytokine with a wide variety of biological functions. It is a potent inducer of the acute phase response. Plays an essential role in the final differentiation of B-cells into Ig-secreting cells Involved in lymphocyte and monocyte differentiation. It induces myeloma and plasmacytoma growth and induces nerve cells differentiation Acts on B-cells, T-cells, hepatocytes, hematopoeitic progenitor cells and cells of the CNS. Also acts as a myokine. It is discharged into the bloodstream after muscle contraction and acts to increase the breakdown of fats and to improve insulin resistance. -
Involvement in disease
Genetic variations in IL6 are associated with susceptibility to rheumatoid arthritis systemic juvenile (RASJ) [MIM:604302]. An inflammatory articular disorder with systemic-onset beginning before the age of 16. It represents a subgroup of juvenile arthritis associated with severe extraarticular features and occasionally fatal complications. During active phases of the disorder, patients display a typical daily spiking fever, an evanescent macular rash, lymphadenopathy, hepatosplenomegaly, serositis, myalgia and arthritis.
Note=A IL6 promoter polymorphism is associated with a lifetime risk of development of Kaposi sarcoma in HIV-infected men. -
Sequence similarities
Belongs to the IL-6 superfamily. -
Post-translational
modificationsN- and O-glycosylated. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- Interleukin BSF 2
- B cell differentiation factor
- B cell stimulatory factor 2
see all -
Database links
- Entrez Gene: 3569 Human
- Omim: 147620 Human
- SwissProt: P05231 Human
- Unigene: 654458 Human
Associated products
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SimpleStep ELISA kits
Images
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Standard curve of human IL-6 with background signal subtracted (duplicates; +/- SD).
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IL-6 measured in cell culture supernatants (diluted 1/50-1/1250) from control or treated (48 hours with 2% PHA-M; LifeTechnologies) PBMCs (duplicates +/- SD).
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IL-6 detected in positive control (Pos CTRL; ab46027), and in supernatants (diluted 1/1-1/5) from control cells or cells stimulated for 24 hours with 50 nanogram x mL-1 of PMA (ab120297) with 1 microgram x mL-1 of LPS (Sigma) for the last 6 hours (PMA/LPS).
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Representative Standard Curve using ab46027
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Dilution curves of human IL-6 (ab46027) and NIBSC standard (89/548). One ng of standard IL-6 corresponds to 87.2 (+/- 8.0) IU NIBSC standard. Background signal subtracted (duplicates; +/- SD).
Datasheets and documents
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SDS download
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Datasheet download
References (76)
ab46027 has been referenced in 76 publications.
- Kameshwar K et al. A pilot study comparing the efficiency of a novel asymmetric cellulose triacetate (ATA) dialyser membrane (Solacea-190H) to a standard high flux polysulfone dialyser membrane (FX-80) in the setting of extended hours haemodialysis. Nephrology (Carlton) 27:494-500 (2022). PubMed: 35195932
- Herrera VLM et al. A targetable 'rogue' neutrophil-subset, [CD11b+DEspR+] immunotype, is associated with severity and mortality in acute respiratory distress syndrome (ARDS) and COVID-19-ARDS. Sci Rep 12:5583 (2022). PubMed: 35379853
- Herrera VLM et al. "Rogue" [DEspR+CD11b+] neutrophil subset correlates with severity in spontaneous intracerebral hemorrhage. Front Neurol 13:935579 (2022). PubMed: 35959408
- Xu W et al. Alterations in bile acids as metabolic signatures in the patients with human adenovirus type 7 infection. Front Med (Lausanne) 9:896409 (2022). PubMed: 36160124
- Khojah HMJ et al. The Impact of Serum Levels of Vitamin D3 and Its Metabolites on the Prognosis and Disease Severity of COVID-19. Nutrients 14:N/A (2022). PubMed: 36558489