the kit is very sensitive and specific. data shows secrection in a dose response by M1 (MCSF+IFNg) human macrophages differentiated in culture for 3 days.
Regarding speed, it took me about 2hrs to run the assay, very much time saving considering a minimum of 5hrs for the standard ELISA. Money wise, price is more or less the same as a standard ELISA. Something interesting is the volume required is 50µl versus the 100µl of a standard ELISA.
Abcam user community
Submitted Jan 20 2014
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