Human IgE full length protein (Azide-free) (ab65866)

Overview

  • Product name
    Human IgE full length protein (Azide-free)
    See all IgE proteins and peptides
  • Protein length
    Full length protein
  • Description
    Native Human IgE protein (Azide free)

Description

  • Nature
    Native
  • Source
    Native
  • Amino Acid Sequence
    • Species
      Human
    • Additional sequence information
      Observed band of ~185kDa on SDS-PAGE

Specifications

Our Abpromise guarantee covers the use of ab65866 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

  • Applications

    SDS-PAGE

    ELISA

    Immunocytochemistry

  • Endotoxin level
    < 25.000 Eu/mg
  • Purity
    > 90 % SDS-PAGE.
    Human IgE full length protein was purified by Protein L chromatography. As the IgE comes from a monoclonal cell line, there is no contamination of antibodies of other isotypes. The remaining contaminants are mainly components from Foetal Bovine Serum.
  • Form
    Liquid
  • Additional notes

    This product is purified Human IgE with kappa light chains produced in vitro from a monoclonal hybridoma.

    This antigen is quite unique, because it is the only available IgE product, which originates from a human monoclonal cell line. Our IgE product shows superior advantage when compared to the traditionally purified polyclonal IgE from myeloma patients. A comparison which is highly superior on the following key parameters:.

    • Less batch-to-batch variation
    • Higher degree of purity (>90%)
    • A more uniform product (monoclonal and not polyclonal)

     

    IgE has been used in a variety of cell stimulation assays, including the following cell types:

    • Human airway smooth muscle (HASM) cells
    • mast cells (MC)
    • Human bronchial/tracheal smooth muscle (B/TSM) cells
    • acute myelogenous leukemia (AML) blasts
    • Human neutrophils

     

    1 mg of ab65866 is 380,000± 50,000 IU/ml.

  • Concentration information loading...

Preparation and Storage

  • Stability and Storage

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.

    pH: 7.40
    Constituents: 0.0268% PBS, 0.87% Sodium chloride

General Info

  • Alternative names
    • Igh2
    • IGHE
    • IGHEP1
    • Immunoglobulin heavy constant epsilon
  • Relevance
    IgE is the class of antibodies produced in the lungs, skin, and mucous membranes. It may protect against parasite invasion, but it is a major factor in allergic reactions. The antigen-specific IgE interacts with mast cells and eosinophils, triggers the release of histamine, leukotrienes and other substances that lead to the itching, sneezing and congestion of allergies - and the life threatening respiratory distress of asthma and anaphylactic shock.
  • Cellular localization
    Cell Membrane

Images

  • Background-subtracted data values (mean +/- SD) are graphed.

  • SDS-PAGE analysis of:

    Lane 1: Molecular weight markers.
    Lane 2: ab65866.
    Lane 3: IgE purified from serum from a myeloma patient.

References

This product has been referenced in:
  • Vidal M  et al. Development of quantitative suspension array assays for six immunoglobulin isotypes and subclasses to multiple Plasmodium falciparum antigens. J Immunol Methods N/A:N/A (2018). Read more (PubMed: 29397157) »
  • Wan Z  et al. Photocleavage-based affinity purification of biomarkers from serum: Application to multiplex allergy testing. PLoS One 13:e0191987 (2018). Read more (PubMed: 29389948) »

See all 7 Publications for this product

Customer reviews and Q&As

We can confirm that the human IgE have kappa light chains.

11-11 of 11 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up