Human KLK5 ELISA Kit (ab131555)
Key features and details
- Sensitivity: 10 pg/ml
- Range: 78 pg/ml - 5000 pg/ml
- Sample type: Cell culture supernatant, Milk, Plasma, Saliva, Serum
- Detection method: Colorimetric
- Assay type: Sandwich (qualitative)
- Reacts with: Human
Overview
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Product name
Human KLK5 ELISA Kit -
Detection method
Colorimetric -
Sample type
Cell culture supernatant, Saliva, Milk, Serum, Plasma -
Assay type
Sandwich (qualitative) -
Sensitivity
< 10 pg/ml -
Range
78 pg/ml - 5000 pg/ml -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s Human KLK 5 in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Human KLK in cell culture supernatants, serum, plasma (heparin , EDTA), human milk and saliva.
A KLK5 specific mouse monoclonal antibody has been precoated onto 96-well plates. Standards and test samples are added to the wells and incubated. A biotinylated detection polyclonal antibody from goat specific for KLK5 is then added followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with PBS or TBS buffer. TMB is then used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the Human KLK5 amount of sample captured in plate.
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components Identifier 1 x 96 tests ABC Diluent Buffer Blue Cap 1 x 12ml Antibody Diluent Buffer Green Cap 1 x 12ml Anti-Human KLK5 antibody Microplate (12 x 8 wells) 1 unit Avidin-Biotin-Peroxidase Complex (ABC) 1 x 100µl Biotinylated anti-Human KLK5 antibody 1 x 100µl Lyophilized recombinant Human KLK5 standard 2 x 10ng Plate Seal 1 x 4 units Sample Diluent Buffer Green Cap 1 x 30ml TMB Color Developing Agent Amber Bottle 1 x 10ml TMB Stop Solution Yellow Cap 1 x 10ml Wash Buffer (25X) 1 x 20ml -
Research areas
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Relevance
May be involved in desquamation. -
Cellular localization
Secreted -
Alternative names
- Kallikrein 5
- Kallikrein-like protein 2
- kallikrein-related peptidase 5
see all -
Database links
- Entrez Gene: 25818 Human
- Omim: 605643 Human
- SwissProt: Q9Y337 Human
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab131555 has not yet been referenced specifically in any publications.