Overview

  • Product name
    Human Osteoactivin ELISA Kit
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall < 10%
    Inter-assay
    Sample n Mean SD CV%
    Overall < 12%
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    45 pg/ml
  • Range
    45 pg/ml - 12000 pg/ml
  • Recovery

    Sample specific recovery
    Sample type Average % Range
    Serum 117.8 111% - 126%
    Plasma 116.5 107% - 126%
    Cell culture media 112.9 98% - 149%

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Osteoactivin Human ELISA Kit (ab193711) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Osteoactivin in sera, plasma and cell culture supernatants.

    This assay employs an antibody specific for Human Osteoactivin coated on a 96-well plate. Standards and samples are pipetted into the wells and the immobilized antibody captures Osteoactivin present in the samples. The wells are washed and biotinylated anti-Human Osteoactivin antibody is added. After washing away any unbound biotinylated antibody, an HRP-conjugated streptavidin is pipetted to the wells. After incubation, the wells are again washed, followed by the addition of a TMB substrate solution to the wells. Color will develop in proportion to the amount of Osteoactivin bound in each well. Addition of the Stop Solution will change the color from blue to yellow, and the intensity of the color is measured at 450 nm.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Pre-coated microplate (12 x 8 well strips)

Properties

  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    20X Wash Buffer Concentrate 1 x 25ml
    5X Assay Diluent B 1 x 15ml
    500X HRP-Streptavidin Concentrate 1 x 200µl
    Assay Diluent A 1 x 30ml
    Biotinylated Human Osteoactivin detection antibody (lyophilized) 2 vials
    Human Osteoactivin Standard (lyophilized) 2 vials
    Pre-coated Human Osteoactivin Microplate (12 strips x 8 wells) 1 unit
    Stop Solution 1 x 8ml
    TMB One-Step Substrate Reagent 1 x 12ml
  • Function
    Could be a melanogenic enzyme.
  • Tissue specificity
    Up-regulated in various cancer cells, including in glioblastoma multiforme. Expressed in many melanoma cells, as well as in tissue macrophages, including liver Kuppfer cells and lung alveolar macrophages, in podocytes and in some cells of the ciliary body of the eye (at protein level). Hardly detectable in healthy brain.
  • Involvement in disease
    Glioblastoma multiforme patients that exhibit increased mRNA and protein levels (>3-fold over normal brain) in biopsy samples have a significantly higher risk of death (PubMed:16609006). In the context of cancer immunotherapy, including that of melanoma, has been proposed to be used as a cell surface protein antigen targeted by antibodies coupled to cytotoxic drugs (PubMed:16489096).
  • Sequence similarities
    Belongs to the PMEL/NMB family.
    Contains 1 PKD domain.
  • Cellular localization
    Cell membrane. Melanosome. Identified by mass spectrometry in melanosome fractions from stage I to stage IV. Detected at the cell surface in different types of cancers cells, including glioblastoma multiforme cells and most melanoma cell lines.
  • Information by UniProt
  • Alternative names
    • Gpnmb
    • GPNMB_HUMAN
    • Transmembrane glycoprotein HGFIN
    • Transmembrane glycoprotein NMB
  • Database links

Applications

Our Abpromise guarantee covers the use of ab193711 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.

  • Data provided for demonstration purposes only. A new standard curve must be generated for each assay performed.

Protocols

References

ab193711 has not yet been referenced specifically in any publications.

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