Human Osteopontin ELISA Kit (ab100618)
Key features and details
- Sensitivity: 50 pg/ml
- Range: 74.07 pg/ml - 18000 pg/ml
- Sample type: Cell culture supernatant, Milk, Plasma, Serum, Urine
- Detection method: Colorimetric
- Assay type: Sandwich (quantitative)
- Reacts with: Human
Overview
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Product name
Human Osteopontin ELISA Kit
See all Osteopontin kits -
Detection method
Colorimetric -
Sample type
Cell culture supernatant, Milk, Urine, Serum, Plasma -
Assay type
Sandwich (quantitative) -
Sensitivity
< 50 pg/ml -
Range
74.07 pg/ml - 18000 pg/ml -
Recovery
95 %
Sample specific recovery Sample type Average % Range Cell culture supernatant 103.5 95% - 118% Serum 89.92 88% - 108% Plasma 92.41 90% - 109% -
Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s Osteopontin Human ELISA (Enzyme-Linked Immunosorbent Assay) kit is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human Osteopontin in serum, plasma, and cell culture supernatants.
This assay employs an antibody specific for Human Osteopontin coated on a 96-well plate. Standards and samples are pipetted into the wells and Osteopontin present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human Osteopontin antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of Osteopontin bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Get higher sensitivity in only 90 minutes with Human Osteopontin ELISA Kit (ab192143) from our SimpleStep ELISA® range.
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Notes
Optimization may be required with urine samples
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Platform
Microplate
Properties
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Storage instructions
Store at -20°C. Please refer to protocols. -
Components 1 x 96 tests 20X Wash Buffer 1 x 25ml 500X HRP-Streptavidin Concentrate 1 x 200µl 5X Assay Diluent B 1 x 15ml Assay Diluent A 1 x 30ml Biotinylated anti-Human Osteopontin 2 vials Osteopontin Microplate (12 x 8 wells) 1 unit Recombinant Human Osteopontin Standard (lyophilized) 2 vials Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml -
Research areas
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Function
Binds tightly to hydroxyapatite. Appears to form an integral part of the mineralized matrix. Probably important to cell-matrix interaction.
Acts as a cytokine involved in enhancing production of interferon-gamma and interleukin-12 and reducing production of interleukin-10 and is essential in the pathway that leads to type I immunity. -
Tissue specificity
Bone. Found in plasma. -
Sequence similarities
Belongs to the osteopontin family. -
Post-translational
modificationsExtensively phosphorylated on clustered serine residues.
N- and O-glycosylated.
Phosphorylation sites are present in the extracelllular medium. -
Cellular localization
Secreted. - Information by UniProt
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Alternative names
- BNSP
- Bone sialoprotein 1
- Bone sialoprotein I
see all -
Database links
- Entrez Gene: 6696 Human
- Omim: 166490 Human
- SwissProt: P10451 Human
- Unigene: 313 Human
Images
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Osteopontin measured in biological fluids showing quantity (pg) per mL of tested sample. Human serum diluted 10-30 fold. Human plasma diluted 50 fold - below detection. Human milk diluted 18000-54000 fold. Human urine diluted 100 fold.
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Representative Standard Curve using ab100618.
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Representative Standard Curve using ab100618.
Datasheets and documents
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SDS download
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Datasheet download
References (15)
ab100618 has been referenced in 15 publications.
- Nawaz SS et al. Determinant of Osteopontin Levels in Microvascular Complications in Patients with Diabetes. Int J Gen Med 15:4433-4440 (2022). PubMed: 35509601
- Fernández-Lizárraga M et al. Evaluation of the Biocompatibility and Osteogenic Properties of Metal Oxide Coatings Applied by Magnetron Sputtering as Potential Biofunctional Surface Modifications for Orthopedic Implants. Materials (Basel) 15:N/A (2022). PubMed: 35955174
- Granata S et al. Sphingomyelin and Medullary Sponge Kidney Disease: A Biological Link Identified by Omics Approach. Front Med (Lausanne) 8:671798 (2021). PubMed: 34124100
- Gaude E et al. A Novel Combination of Blood Biomarkers and Clinical Stroke Scales Facilitates Detection of Large Vessel Occlusion Ischemic Strokes. Diagnostics (Basel) 11:N/A (2021). PubMed: 34206615
- Han Z et al. Relationship between miRNA-433 and SPP1 in the presence of fracture and traumatic brain injury. Exp Ther Med 22:928 (2021). PubMed: 34306197