Overview

  • Product name
    Human PMN Elastase ELISA Kit
    See all Neutrophil Elastase kits
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall 4.8%
    Inter-assay
    Sample n Mean SD CV%
    Overall 5.6%
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    1.98 pg/ml
  • Range
    0.16 ng/ml - 10 ng/ml
  • Recovery

    104 %

    Sample specific recovery
    Sample type Average % Range
    Plasma 104 96% - 110%

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s PMN Elastase Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for accurate quantitative measurement of Human PMN Elastase concentrations in Cell culture supernatants, plasma, exudate, bronchoalveolar lavage fluid, cerebrospinal fluid and seminal plasma.


    PMN Elastase specific antibodies have been precoated onto 96-well plates. Standards and test samples are added to the wells and along with an HRP-conjugated PMN Elastase detection antibody and the microplate is then incubated at room temperature. After the removal of unbound proteins by washing, TMB is used to visualize the HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is directly proportional to the PMN Elastase amount of sample captured in plate. 


    The capture antibody in the Human PMN-Elastase ELISA kit is specific to PMN elastase, while the detection antibody is specific to alpha1-PI, hence the kit is specific to the PMN Elastase/alpha1-PI complex.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform
    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab119553 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.

Images

  • Standard curve with background signal subtracted (duplicates; +/- SD).

  • PMN elastase measured in the high control and low control (ab119553) and in biological fluids (dilution range 1/1-1/1000; duplicates +/- SD).

  • Representative Standard Curve using ab119553.

Protocols

References

This product has been referenced in:
  • Thålin C  et al. Citrullinated histone H3 as a novel prognostic blood marker in patients with advanced cancer. PLoS One 13:e0191231 (2018). ELISA ; Human . Read more (PubMed: 29324871) »
  • Schechter MC  et al. Neutrophil extracellular trap (NET) levels in human plasma are associated with active TB. PLoS One 12:e0182587 (2017). ELISA ; Human . Read more (PubMed: 28777804) »
See all 7 Publications for this product

Customer reviews and Q&As

1-10 of 13 Abreviews or Q&A

Answer

Fresh plasma samples are excellent ELISA samples however 1 time frozen and thawed should be fine as well. Generally, freeze thaw 3-4 times is very detrimental for plasma/serum proteins therefore we suggest avoiding multiple freeze thawing.
In case you see NE concentration lower than expected then I would recommend using fresh samples theoretically, the one freeze thaw step should be absolutely fine.  

Read More

Question
Answer

The PMN elastase ELISA ab119553 has not, to our knowledge, been used to test sputum samples, but it will in theory be compatible with that sample type. The question is whether the assay will be sensitive enough to detect the amounts of elastase present in sputum, and also whether the elastase epitopes that the antibodies in the ELISA detect are intact, and not degraded by proteases.

Read More

Elastase levels in gut mucosa lysates

Excellent Excellent 5/5 (Ease of Use)
Abreviews
Followed the kit's protocol from start to finish.
The samples were whole tissue lysates of human gut mucosa which were assayed in various dilutions (in PBS). Have not tried any other sample type.
Kit is very easy to use and works just like any standard ELISA.
Got consitently good results. Will buy it again.

Abcam user community

Verified customer

Submitted Mar 13 2014

Answer


This kit works with plasma treated with EDTA or citrate.
We have no information pertaining to the use of other anti-coagulants in use with this kit.

Read More

Answer

I am sorry for the delay in getting back to you.

I now have further information to share with you in regards to the Neutrophil Elastase ELISA you are hoping to set up.

If you would be interested in setting up your own ELISA I have looked up the following components that should work together to work in an indirect ELISA:

Primary antibody:

Rabbit polyclonal anti-Neutrophil Elastase antibody (ab21595)

Secondary antibody:

Goat polyclonal Secondary antibody to Rabbit IgG - H&L, pre-adsorbed, HRP conjugated (ab97080)

Protein standard:

Neutrophil Elastase protein (Active) (ab91099)

These three products come to a total of £616.

Although we have not used this particular combination in an ELISA, and the protein has not been used in an ELISA at all, I would expect it to work together. However, as we have not tried it I would not be able to guarantee it. I would however be able to offer you a testing discount as this is the case. This would mean that if you purchase ab91099 as normal, test it in an ELISA, and let us know the results through an Abreview (no matter whether the results are positive or negative) you would be eligible to receive the value of ab91099 off any future purchase with Abcam. The only constraint to this offer is that the "free" product must be claimed within 4 months of purchasing ab91099. More information on this offer can be found form the following link:

https://www.abcam.com/abtrial

Please let me know if you would be interested in taking part in this scheme as a discount code needs to be generated prior to purchasing ab91099.

I am currently in contact with the source of the primary antibody in order to obtain the protocol used in ELISA with this antibody. Once I have this information I will let you know.

Read More

Answer

Thank you for contacting us and your interest in our products.

I am sorry but having looked into it, we are unfortunately not able to provide the standard or high/low samples for the PMN Elastase Human ELISA kit (ab119553) separately.

We do however have a number of protein preparations which may be of interest to you to serve as standards in your assay. These can be accessed from the following link:

https://www.abcam.com/index.html?pageconfig=searchresults&search=Neutrophil Elastase&pt=2&sk=targ&sv=Neutrophil Elastase&sn=Neutrophil Elastase&l=2&fViewMore=1

If you would like any assistance in choosing which protein may be suitable please could you provide a little more information about your experiment? The antibodies you hope to use with it?

I hope this information has been of help. If I can be of any further assistance, please do let me know.

Read More

Answer

Thank you for contacting us.
Unfortunately, it is not possible to obtain the standard and detection antibody separately for this kit. Also, the coating antibody is bound on the plates and is not available separately either.
I am sorry to not be able to help you more in this case.

We carry however neutrophil elastase antibodies that have been tested in indirect ELISA: ab21595, ab41179, ab14188, and ab37713. Please let me know if you would be interested in one of these.
We also carry neutrophil elastase proteins: ab91099, ab80475 and a peptide ab68671.
Please do not hesitate to contact us if you need any more advice or information.
Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

Read More

Answer

Thank you for contacting Abcam regarding ab119553.


I can confirm that this kit will only recognize the complex of PMN elastase/alpha 1-PI complex standards and also our standard is lyophilized complex.


I hope this information is helpful. Please do not hesitate to contact us if you have any additional questions.

Read More

Answer

Ihave issued a freekit, 8-hydroxy 2 deoxyguanosine ELISA Kit, ab101245,with the order number 1070428.

To check the status of the order please contact our Customer Service team and reference this number.

Again, I apologize for any delay in your research. Please contact me if you need anything further.

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1-10 of 13 Abreviews or Q&A

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