Overview

  • Product name
    Anti-Human Papillomavirus 16 (E7) antibody [289-17013]
    See all Human Papillomavirus 16 (E7) primary antibodies
  • Description
    Mouse monoclonal [289-17013] to Human Papillomavirus 16 (E7)
  • Host species
    Mouse
  • Tested applications
    Suitable for: IP, WB, ELISA, RIA, Flow Cyt, IHC-Frmore details
  • Species reactivity
    Reacts with: Other species
  • Immunogen

    Full length protein corresponding to Human Papillomavirus 16 (E7). Immunogen: HPV 16 E7 synthesized in S. pombe.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer
    pH: 7.40
    Preservative: 0.05% Sodium azide
    Constituents: 0.87% Sodium chloride, PBS
  • Concentration information loading...
  • Purity
    Ascites
  • Purification notes
    Purified from ascites.
  • Clonality
    Monoclonal
  • Clone number
    289-17013
  • Isotype
    IgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab30731 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent concentration.
WB Use at an assay dependent concentration. Predicted molecular weight: 11 kDa. Dilution optimised using Chromogenic detection.
ELISA Use at an assay dependent concentration.
RIA Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody.

IHC-Fr Use at an assay dependent concentration.

Target

  • Relevance
    E7 protein has both transforming and trans-activating activities. Disrupts the function of host retinoblastoma protein RB1/pRb, which is a key regulator of the cell cycle. Induces the disassembly of the E2F1 transcription factors from RB1, with subsequent transcriptional activation of E2F1-regulated S-phase genes. Inactivation of the ability of RB1 to arrest the cell cycle is critical for cellular transformation, uncontrolled cellular growth and proliferation induced by viral infection. Stimulation of progression from G1 to S phase allows the virus to efficiently use the cellular DNA replicating machinery to achieve viral genome replication. Interferes with histone deacetylation mediated by HDAC1 and HDAC2, leading to activation of transcription
  • Cellular localization
    Nuclear
  • Alternative names
    • E7 antibody
    • HPV16 E7 antibody
    • Protein E7 antibody
    • Transforming protein antibody

References

This product has been referenced in:
  • Liu Y  et al. Nuclear lactate dehydrogenase A senses ROS to produce a-hydroxybutyrate for HPV-induced cervical tumor growth. Nat Commun 9:4429 (2018). Read more (PubMed: 30356100) »
  • Mitkevich VA  et al. Binase treatment increases interferon sensitivity and apoptosis in SiHa cervical carcinoma cells by downregulating E6 and E7 human papilloma virus oncoproteins. Oncotarget 8:72666-72675 (2017). Read more (PubMed: 29069817) »
See all 5 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

Thank you for your enquiry and your interest in our products.

These two monoclonal antibodies recognize the same protein (Human Papillomavirus 16 (E7)) but are from different clones.

https://www.abcam.com/ab20191:

https://www.abcam.com/ab30731:

As you can see on the datasheets, the concentration, the tested application and the storage buffer are different.

If you need any further assistance in the future, please do not hesitate to contact me.

Read More

Answer

We are not aware of publications having used ab30731.

Read More

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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