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|Sample type||Average %||Range|
|Cell culture supernatant||97.87||85% - 104%|
|Serum||94.46||82% - 102%|
|Plasma||95.78||93% - 103%|
Human TGF beta 1 ELISA (Enzyme-Linked Immunosorbent Assay) kit (ab100647) is an in vitro enzyme-linked immunosorbent assay for the quantitative measurement of Human TGF beta 1 in serum, plasma and cell culture supernatants.
This assay employs an antibody specific for Human TGF beta 1 coated on a 96-well plate. Standards and samples are pipetted into the wells and TGF beta 1 present in a sample is bound to the wells by the immobilized antibody. The wells are washed and biotinylated anti-Human TGF beta 1 antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of TGF beta 1 bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
Ab100647 was reformulated on 31st May 2018 with new capture and detector antibodies that allows to increase the sensitivity to human TGF beta 1.
Please note that as a consequence of this change, the procedure and protocol have slightly changed and we encourage you to review the protocol steps before starting your experiments
Optimization may be required with urine samples.
|Components||1 x 96 tests|
|20X Wash Buffer Concentrate||1 x 25ml|
|500X HRP-Streptavidin Concentrate||1 x 200µl|
|5X Assay Diluent||1 x 15ml|
|Biotinylated anti-Human TGF beta 1 (lyophilized)||2 vials|
|Recombinant Human TGF beta 1 Standard (lyophilized)||2 vials|
|Stop Solution||1 x 8ml|
|TGF beta 1 Microplate (12 x 8 wells)||1 unit|
|TMB One-Step Substrate Reagent||1 x 12ml|
Our Abpromise guarantee covers the use of ab100647 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|Sandwich ELISA||Use at an assay dependent concentration.|
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"