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The Assay Diluent B 5x was cloudy upon arrival. Is this normal or is the sample contaminated?
Asked on Apr 19 2012
Thank you for calling Abcam earlier today.
I have talked to the lab and they have told me that Assay Diluent B does contain some protein and solutes so it would not be uncommon to see a little cloudiness or floating unsolubilized protein aggregates (especially after storing at low temperatures and/or for a long period of time). Most of the time bringing the solution up to room temperature, mixing it thoroughly and diluting to the 1X working concentration will help clarify the solution and solubilize any protein aggregates or precipitates. Centrifuging and filtering the solution can also help but usually doing so is not needed.
Please let me know if there is anything else I can help you with.
Answered on Apr 19 2012