• Product name
    Human TNF alpha ELISA Kit
    See all TNF alpha kits
  • Detection method
  • Precision
    Sample n Mean SD CV%
    Sample 6 3.3%
    Sample n Mean SD CV%
    Sample 6 9%
  • Sample type
    Cell culture supernatant, Serum, Plasma
  • Assay type
    Sandwich (quantitative)
  • Sensitivity
    < 10 pg/ml
  • Range
    25 pg/ml - 800 pg/ml
  • Recovery

    107 %

    Sample specific recovery
    Sample type Average % Range
    Serum 81 74% - 90%

  • Assay time
    3h 40m
  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s TNF alpha Human in vitro ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the quantitative measurement of TNF alpha in supernatants, buffered solutions, serum and plasma samples.

    A monoclonal antibody specific TNF alpha has been coated onto the wells of the microtiter strips provided. Samples, including standards of known TNF alpha concentrations, control specimens or unknowns are pipetted into these wells. During the first incubation, the standards or samples and a biotinylated monoclonal antibody specific for TNF alpha are simultaneously incubated. After washing, the enzyme Streptavidin-HRP, that binds the biotinylated antibody is added, incubated and washed. A TMB substrate solution is added which acts on the bound enzyme to induce a colored reaction product. The intensity of this colored product is directly proportional to the concentration of TNF alpha present in the samples. 

    This kit will recognize both endogenous and recombinant Human TNF alpha.

    Get results in 90 minutes with Human TNF alpha ELISA Kit (ab181421) from our SimpleStep ELISA® range.

  • Tested applications
    Suitable for: Sandwich ELISAmore details
  • Platform


  • Storage instructions
    Store at +4°C. Please refer to protocols.
  • Components Identifier 1 x 96 tests 2 x 96 tests
    10X Standard Diluent Buffer Black 1 x 25ml 1 x 25ml
    Wash Buffer White 1 x 10ml 2 x 10ml
    Biotinylated Antibody Diluent Red 1 x 7.5ml 1 x 13ml
    Biotinylated anti-TNF alpha Red 1 x 400µl 2 x 400µl
    TMB Substrate 1 x 11ml 1 x 24ml
    TNF alpha Control Yellow 2 vials 4 vials
    HRP Diluent Red 1 x 23ml 1 x 23ml
    Plastic Plate Covers 1 x 2 units 1 x 4 units
    Standard Diluent (Human Serum) 1 x 7ml 2 x 7ml
    Stop Reagent Black 1 x 11ml 2 x 11ml
    Streptavidin-HRP 2 x 5µl 4 x 5µl
    TNF alpha Microplate (12 x 8 well strips) 1 unit 2 units
    TNF alpha Standard 2 vials 4 vials
  • Research areas
  • Function
    Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation.
  • Involvement in disease
    Genetic variations in TNF are a cause of susceptibility psoriatic arthritis (PSORAS) [MIM:607507]. PSORAS is an inflammatory, seronegative arthritis associated with psoriasis. It is a heterogeneous disorder ranging from a mild, non-destructive disease to a severe, progressive, erosive arthropathy. Five types of psoriatic arthritis have been defined: asymmetrical oligoarthritis characterized by primary involvement of the small joints of the fingers or toes; asymmetrical arthritis which involves the joints of the extremities; symmetrical polyarthritis characterized by a rheumatoidlike pattern that can involve hands, wrists, ankles, and feet; arthritis mutilans, which is a rare but deforming and destructive condition; arthritis of the sacroiliac joints and spine (psoriatic spondylitis).
  • Sequence similarities
    Belongs to the tumor necrosis factor family.
  • Post-translational
    The soluble form derives from the membrane form by proteolytic processing.
    The membrane form, but not the soluble form, is phosphorylated on serine residues. Dephosphorylation of the membrane form occurs by binding to soluble TNFRSF1A/TNFR1.
    O-glycosylated; glycans contain galactose, N-acetylgalactosamine and N-acetylneuraminic acid.
  • Cellular localization
    Secreted and Cell membrane.
  • Information by UniProt
  • Alternative names
    • APC1 protein
    • Cachectin
    • DIF
    • soluble form
    • Tnf
    • TNF a
    • TNF macrophage derived
    • TNF monocyte derived
    • TNF superfamily member 2
    • TNF-a
    • TNF-alpha
    • TNFSF2
    • Tumor necrosis factor
    • Tumor necrosis factor alpha
    • Tumor necrosis factor ligand superfamily member 2
    see all
  • Database links

Associated products


Our Abpromise guarantee covers the use of ab46087 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA Use at an assay dependent concentration.


  • Representative standard curve using ab46087

  • TNF? was measured in cell culture medium from U937 control cells, or cells stimulated with LPS (1 �g x mL-1; 6 h) or PMA (10 ng x mL-1; 24 h) with LPS and control and stimulated PBMCs (PHA 10 �g x mL-1; 3 days). Results are shown with background signal subtracted (duplicates +/- SD), with the positive control from ab46087 included.



This product has been referenced in:
  • Ren X  et al. Effects of fluconazole on the clinical outcome and immune response in fungal co-infected tuberculosis patients. Microb Pathog 117:148-152 (2018). ELISA . Read more (PubMed: 29432913) »
  • Zhao L  et al. Chitosan oligosaccharide improves the therapeutic efficacy of sitagliptin for the therapy of Chinese elderly patients with type 2 diabetes mellitus. Ther Clin Risk Manag 13:739-750 (2017). Read more (PubMed: 28721055) »

See all 5 Publications for this product

Customer reviews and Q&As

The specification 1,16 ml on the label of the TNF alpha standard means 1.16 ml. (The volume will vary by lot). Using a comma for the decimal mark is conventional in many countries in Europe, where the standard is produced. If you are interested, the Wi...

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Vielen Dank für Ihre Anfrage.

Ich kann Ihnen bestätigen, dass beide Kits für die Analyse von humanem TNF alpha in Zellkultur Überstand, Serum, Plasma getestet und garantiert sind. Beide haben individuelle Strips zum Heraus...

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Thank you for contacting us.

The lab informed me that saliva samples are suitable with the ELISA kit ab46087. There is no particular recommendation to add. The standard protocol can be used.

I hope this information is helpful to y...

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Thank you very much for your reply.

I am glad that you have found some kits that will be suitable for your assay. If you need any help standardizing your methods, please let me know and I'll be happy to assist.

Have a wonderful day.

Thank you very much for contacting us with your questions, and for your patience while I have been in touch with the lab regarding your enquiry.

For the TAC kit ab65329, you can use a general lysis buffer such as RIPA to prepare the lysates.T...

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Thank you for calling earlier, it was a pleasure speaking with you.

As we discussed on the phone, the standard for the TNF alpha kit is available separately as ab129028 (I've included a link to the datasheet in this email).

Have a gr...

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Thank you for contacting us.

Yes, the lab confirmed thatab129028 can be used together with the kit ab46087.

We will update our datasheets to reflect that information.

I hope this information is helpful to you. Please do n...

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Thank you for contacting Abcam.

I have talked to the abID and we should be able to add the biotinylated antibody from an46087 to the catalogue. It should be available for purchase in a few days. I will email you the abID codes when it has bee...

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Thank you for contacting Abcam earlier today.

I have heard back from the lab already and we are able to add the standards from ab46087 to the catalogue individually. It will take a day or so to have them added to the catalogue and I will cont...

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Thank you for your enquiry and interest.

I can confirm that this product (TNF alpha Human ELISA Kit) has been characterized on cell culture supernatant, serum, plasma. It has not been tested specifically in saliva; however as long as the samp...

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1-10 of 18 Abreviews or Q&A


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