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thank you for your mail, which, however, did not answer my question: why measuring VEGF in cell extracts instead of measuring it in supernatants? to measure also the membrane anchored VEGF? but in this case (ie, protein supposed to increase the levels of the latter forms), is it not better optimizing to see just these forms? Are there other scientific explanations? as I told you, I intend to buy a kit and, as you commercialize both types of kits, thought you would have a clear answer to help me choose what is best for my experiments. Thank you in advance. Yours sincerely,
Asked on Nov 18 2011
Thank you for contacting us. We unfortunately are not aware about the purpose of assaying VEGF in cell supernatant or in cell/tissue culture lysates. You may need to search literature to know more about this. The following publication will help to start. http://www.ncbi.nlm.nih.gov/pubmed/1380254 VEGF 165, 121 are the secreted protein and 189 and 206 are the membrane bound proteins so it will be interesting to know the total amount of VEGF in cell culture samples (supernatant + lysates) however it will depend on the type of experiment and results you will be expecting. Ab100662 is a best kit for detection of VEGF in cell/tissue culture lysates while ab100663 is best for VEGF detection in serum, plasma or cell culture supernatant. I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.
Abcam Scientific Support
Answered on Nov 18 2011